DNA-free directed assembly in single-molecule cut-and-paste.
Sign Up to like & getrecommendations! Published in 2019 at "Nanoscale"
DOI: 10.1039/c8nr08636b
Abstract: Single-molecule cut-and-paste facilitates bottom-up directed assembly of nanoscale biomolecular networks in defined geometries and enables analysis with spatio-temporal resolution. However, arrangement of diverse molecules of interest requires versatile handling systems. The novel DNA-free, genetically encodable… read more here.
Keywords: single molecule; molecule cut; directed assembly; cut paste ... See more keywords
A switchable DNA origami/plasmonic hybrid device with a precisely tuneable DNA-free interparticle gap.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical communications"
DOI: 10.1039/d2cc05324a
Abstract: We here show a reconfigurable DNA/plasmonic nanodevice with a precisely tunable and DNA-free interparticle gap. The nanodevice comprises two DNA boxes for the size-selective incorporation of nanoparticles in a face-to-face orientation and an underlying switchable… read more here.
Keywords: free interparticle; dna; dna free; interparticle ... See more keywords
DNA-free genome editing for ZmPLA1 gene via targeting immature embryos in tropical maize.
Sign Up to like & getrecommendations! Published in 2023 at "GM crops & food"
DOI: 10.1080/21645698.2023.2197303
Abstract: Doubled haploid (DH) production accelerates the development of homozygous lines in a single generation. In maize, haploids are widely produced by the use of haploid inducer Stock 6, earlier reported in 1959. Three independent studies… read more here.
Keywords: zmpla1 gene; dna free; immature embryos; free genome ... See more keywords
DNA-free CRISPR-Cas9 gene editing of wild tetraploid tomato Solanum peruvianum using protoplast regeneration
Sign Up to like & getrecommendations! Published in 2022 at "Plant Physiology"
DOI: 10.1093/plphys/kiac022
Abstract: Abstract Wild tomatoes (Solanum peruvianum) are important genomic resources for tomato research and breeding. Development of a foreign DNA-free clustered regularly interspaced short palindromic repeat (CRISPR)-Cas delivery system has potential to mitigate public concern about… read more here.
Keywords: crispr cas9; crispr; dna free; peruvianum ... See more keywords
A stable DNA-free screening system for CRISPR/RNPs-mediated gene editing in hot and sweet cultivars of Capsicum annuum
Sign Up to like & getrecommendations! Published in 2020 at "BMC Plant Biology"
DOI: 10.1186/s12870-020-02665-0
Abstract: Background DNA-free, clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein (Cas) ribonucleoprotein (RNP)-based genome editing is a simple, convincing, and promising tool for precision crop breeding. The efficacy of designed CRISPR-based genome editing tools is… read more here.
Keywords: crispr rnps; gene editing; crispr; capsicum annuum ... See more keywords
DNA-Free Genome Editing: Past, Present and Future
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2018.01957
Abstract: Genome Editing using engineered endonuclease (GEEN) systems rapidly took over the field of plant science and plant breeding. So far, Genome Editing techniques have been applied in more than fifty different plants; including model species… read more here.
Keywords: genome editing; free genome; plant; genome ... See more keywords
Regeneration of non-chimeric plants from DNA-free edited grapevine protoplasts
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.1078931
Abstract: The application of New Breeding Techniques (NBTs) in Vitis vinifera is highly desirable to introduce valuable traits while preserving the genotype of the elite cultivars. However, a broad application of NBTs through standard DNA-based transformation… read more here.
Keywords: chimeric plants; dna free; grapevine; edited grapevine ... See more keywords
Generation of Transfer-DNA-Free Base-Edited Citrus Plants
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.835282
Abstract: To recover transgenic citrus plants in the most efficient manner, the use of selection marker genes is essential. In this work, it was shown that the mutated forms of the acetolactate synthase (ALS) gene in… read more here.
Keywords: dna free; generation; edited citrus; citrus plants ... See more keywords