Analysis of Nuclear Uracil DNA-Glycosylase (nUDG) Turnover During the Cell Cycle.
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DOI: 10.1007/978-1-4939-6603-5_11
Abstract: Uracil-DNA glycosylases (UDG/UNG) are enzymes that remove uracil from DNA and initiate base-excision repair. These enzymes play a key role in maintaining genomic integrity by reducing the mutagenic events caused by G:C to A:T transition… read more here.
Keywords: uracil dna; dna; cell cycle; dna glycosylase ... See more keywords
Base excision-initiated terminal deoxynucleotide transferase-assisted amplification for simultaneous detection of multiple DNA glycosylases
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DOI: 10.1007/s00216-022-03978-9
Abstract: Various DNA glycosylases involved in base excision repair may be associated with a wide disease spectrum that includes cancer, myocardial infarction, neurodegenerative disorders, etc. In this paper, we developed a sensitive method for simultaneous detection… read more here.
Keywords: dna; amplification; detection; dna glycosylases ... See more keywords
Biochemical characterization and mutational studies of the 8-oxoguanine DNA glycosylase from the hyperthermophilic and radioresistant archaeon Thermococcus gammatolerans
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DOI: 10.1007/s00253-019-10031-w
Abstract: 8-oxoguanine (GO) is a major lesion found in DNA that arises from guanine oxidation. The hyperthermophilic and radioresistant euryarchaeon Thermococcus gammatolerans encodes an archaeal GO DNA glycosylase (Tg-AGOG). Here, we characterized biochemically Tg-AGOG and probed… read more here.
Keywords: dna; dna glycosylase; mutational studies; hyperthermophilic radioresistant ... See more keywords
Development of a background signal suppression probe for 8-oxoguanine DNA glycosylase detection.
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DOI: 10.1016/j.aca.2021.338741
Abstract: 8-oxoguanine DNA glycosylase (OGG), which plays a crucial role in base excision repair (BER), is an important biomarker. The existing highly sensitive fluorescent methods always need complicated amplification design. The method with high sensitivity and… read more here.
Keywords: oxoguanine dna; background signal; dna glycosylase; probe ... See more keywords
Closing-upon-repair DNA tetrahedron nanoswitch for FRET imaging the repair activity of 8-oxoguanine DNA glycosylase in living cells.
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DOI: 10.1016/j.aca.2022.339481
Abstract: In situ imaging the repair activity of 8-oxoguanine (8-OG) DNA glycosylase in living cells is important as it is associated with genetic mutation. However, the existing imaging methods confront the interference of intracellular nuclease and… read more here.
Keywords: dna; imaging repair; repair; activity ... See more keywords
Use of a molecular beacon based fluorescent method for assaying uracil DNA glycosylase (Ung) activity and inhibitor screening
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DOI: 10.1016/j.bbrep.2021.100954
Abstract: Uracil DNA glycosylases are an important class of enzymes that hydrolyze the N-glycosidic bond between the uracil base and the deoxyribose sugar to initiate uracil excision repair. Uracil may arise in DNA either because of… read more here.
Keywords: use molecular; uracil dna; activity; dna glycosylase ... See more keywords
Human cells contain a factor that facilitates the DNA glycosylase-mediated excision of oxidized bases from occluded sites in nucleosomes.
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DOI: 10.1016/j.dnarep.2017.06.029
Abstract: Reactive oxygen species generate some 20,000 base lesions per human cell per day. The vast majority of these potentially mutagenic or cytotoxic lesions are subject to base excision repair (BER). Although chromatin remodelers have been… read more here.
Keywords: sites nucleosomes; excision; dna glycosylase; oxidized bases ... See more keywords
Residue coevolution reveals functionally important intramolecular interactions in formamidopyrimidine-DNA glycosylase.
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DOI: 10.1016/j.dnarep.2018.07.004
Abstract: In protein evolution, functionally important intramolecular interactions, such as polar bridges or hydrophobic interfaces, tend to be conserved. We have analyzed coevolution of physicochemical properties in pairs of amino acid residues in the formamidopyrimidine-DNA glycosylase… read more here.
Keywords: coevolution; important intramolecular; dna glycosylase; functionally important ... See more keywords
Unhooking of an interstrand cross-link at DNA fork structures by the DNA glycosylase NEIL3.
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DOI: 10.1016/j.dnarep.2019.102752
Abstract: Interstrand DNA-DNA cross-links (ICLs) are generated by endogenous processes, drugs, and environmental toxins. Understanding the cellular pathways by which various ICLs are repaired is critical to understanding their biological effects. Recent studies showed that replication-dependent… read more here.
Keywords: dna; icl; dna glycosylase; fork ... See more keywords
The activity of the DNA repair enzyme hOGG1 can be directly modulated by ubiquinol.
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DOI: 10.1016/j.dnarep.2019.102784
Abstract: The DNA of human cells suffers about 1.000-100.000 oxidative lesions per day. One of the most common defects in this category is represented by 7,8-dihydro-8-oxoguanine. There are numerous exogenous effects on DNA that induce the… read more here.
Keywords: oxoguanine dna; activity; dna glycosylase; dna ... See more keywords
Biochemical characterization and mutational studies of a novel 3-methlyadenine DNA glycosylase II from the hyperthermophilic Thermococcus gammatolerans.
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DOI: 10.1016/j.dnarep.2020.103030
Abstract: The hyperthermophilic and radioresistant euryarchaeon Thermococcus gammatolerans encodes a putative 3-methlyadenine DNA glycosylase II (Tg-AlkA). Herein, we report biochemical characterization and catalytic mechanism of Tg-AlkA. The recombinant Tg-AlkA can excise hypoxanthine (Hx) and 1-methlyadenine (1-meA)… read more here.
Keywords: dna; dna glycosylase; thermococcus; thermococcus gammatolerans ... See more keywords