Multivalent forms of the ribonuclease H1 hybrid binding domain are high‐affinity binders of RNA–DNA hybrids
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DOI: 10.1002/1873-3468.14541
Abstract: The hybrid binding domain (HBD) is a conserved fold present in ribonucleases H1 that selectively recognizes RNA–DNA hybrids, which are structures present in cellular R‐loops and participate in diverse biological processes. We engineered multivalent HBD… read more here.
Keywords: rna dna; hybrid binding; high affinity; affinity ... See more keywords
Pathogenic mutations reveal a role of RECQ4 in mitochondrial RNA:DNA hybrid formation and resolution
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DOI: 10.1038/s41598-020-74095-9
Abstract: The synthesis of mitochondrial DNA (mtDNA) is a complex process that involves the formation and resolution of unusual nucleic acid structures, such as RNA:DNA hybrids. However, little is known about the enzymes that regulate these… read more here.
Keywords: formation resolution; mtdna; dna; rna dna ... See more keywords
Supramolecular assembly of DNA-constructed vesicles.
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DOI: 10.1039/d0nr04103c
Abstract: The self-assembly of DNA hybrids possessing tetraphenylethylene sticky ends at both sides into vesicular architectures in aqueous medium is demonstrated. Cryo-electron microscopy reveals the formation of different types of morphologies from the amphiphilic DNA-hybrids. Depending… read more here.
Keywords: dna; assembly dna; dna hybrids; constructed vesicles ... See more keywords
Elongating RNA polymerase II and RNA:DNA hybrids hinder fork progression and gene expression at sites of head-on replication-transcription collisions
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DOI: 10.1093/nar/gkab1146
Abstract: Abstract Uncoordinated clashes between replication forks and transcription cause replication stress and genome instability, which are hallmarks of cancer and neurodegeneration. Here, we investigate the outcomes of head-on replication-transcription collisions, using as a model system… read more here.
Keywords: replication; dna hybrids; rna polymerase; rna dna ... See more keywords