Large scale genomic rearrangements in selected Arabidopsis thaliana T-DNA lines are caused by T-DNA insertion mutagenesis
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DOI: 10.1186/s12864-021-07877-8
Abstract: Background Experimental proof of gene function assignments in plants is based on mutant analyses. T-DNA insertion lines provided an invaluable resource of mutants and enabled systematic reverse genetics-based investigation of the functions of Arabidopsis thaliana… read more here.
Keywords: arabidopsis thaliana; dna insertion; insertion; dna insertions ... See more keywords
The complex architecture and epigenomic impact of plant T-DNA insertions
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DOI: 10.1371/journal.pgen.1007819
Abstract: The bacterium Agrobacterium tumefaciens has been the workhorse in plant genome engineering. Customized replacement of native tumor-inducing (Ti) plasmid elements enabled insertion of a sequence of interest called Transfer-DNA (T-DNA) into any plant genome. Although… read more here.
Keywords: plant; dna; dna insertions; insertion ... See more keywords
TDNAscan: A Software to Identify Complete and Truncated T-DNA Insertions
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DOI: 10.3389/fgene.2019.00685
Abstract: Transfer (T)-DNA insertions in mutants isolated from forward genetic screens are typically identified through thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR), inverse PCR, or plasmid rescue. Despite the popularity and success of these methods, they… read more here.
Keywords: dna; dna insertions; genetics; truncated dna ... See more keywords
Recombination between T-DNA insertions to cause chromosomal deletions in Arabidopsis is a rare phenomenon
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DOI: 10.7717/peerj.5076
Abstract: We previously described the identification of a chromosomal deletion in Arabidopsis thaliana that resulted in the elimination of genomic DNA between two T-DNA insertions located ca. 25 kilobases apart on chromosome IV. The mechanism responsible… read more here.
Keywords: recombination dna; dna; dna insertions; deletion ... See more keywords