A protocol for good quality genomic DNA isolation from formalin-fixed paraffin-embedded tissues without using commercial kits.
Sign Up to like & getrecommendations! Published in 2022 at "Molecular biology reports"
DOI: 10.1007/s11033-022-07394-1
Abstract: BACKGROUND DNA isolation from formalin-fixed paraffin-embedded (FFPE) tissues for molecular analysis has become a frequent procedure in cancer research. However, the yield or quality of the isolated DNA is often compromised, and commercial kits are… read more here.
Keywords: dna; commercial kits; dna isolation; isolation formalin ... See more keywords
Silica-Coated Iron Oxide Nanoparticles for DNA Isolation for Molecular Genetic Studies in Hematology.
Sign Up to like & getrecommendations! Published in 2021 at "Genetic testing and molecular biomarkers"
DOI: 10.1089/gtmb.2021.0067
Abstract: Aim: To develop magnetic nanoparticles (MNPs) based on iron oxide for DNA isolation from blood cells for quantitative molecular genetic analyses of the V617F mutation in the Januskinase 2 (JAK2) gene. Materials and Methods: MNPs… read more here.
Keywords: dna isolation; hematology; molecular genetic; genetic studies ... See more keywords
DNA isolation methods for Nanopore sequencing of the Streptococcus mitis genome
Sign Up to like & getrecommendations! Published in 2022 at "Microbial Genomics"
DOI: 10.1099/mgen.0.000764
Abstract: Streptococcus mitis is a Gram-positive bacterium, member of the oral commensal microbiota, which can occasionally be the etiologic agent of diseases such as infective endocarditis, bacteraemia and septicaemia. The highly recombinogenic and repetitive nature of… read more here.
Keywords: dna; mitis; dna isolation; nanopore sequencing ... See more keywords
DNA Isolation and Optimization of ISSR-PCR Reaction System in Oryza sativa L.
Sign Up to like & getrecommendations! Published in 2017 at "International Journal on Advanced Science, Engineering and Information Technology"
DOI: 10.18517/ijaseit.7.6.1621
Abstract: Inter simple sequence repeats (ISSRs) have been utilized widely for molecular markers in analyzing the genetic diversity and phylogenetic and regions in the genome flanked by microsatellite sequences. PCR amplification of these regions using a… read more here.
Keywords: issr; oryza sativa; dna isolation; issr primers ... See more keywords
Comparison of Three Different DNA Isolation Methods To Degradate The Trichoderma Fungi Cell Wall
Sign Up to like & getrecommendations! Published in 2019 at "Bioscience"
DOI: 10.24036/0201931102859-0-00
Abstract: Trichoderma fungi cell walls are composed of chitin compounds that are very sturdy and resistant to enzyme activity. The specific techniques and methods are needed to degradate of fungi cell wall. This study aims to… read more here.
Keywords: dna isolation; dna; dneasy plant; isolation ... See more keywords
A Simplified and Efficient Method for Production of Manganese Ferrite Magnetic Nanoparticles and Their Application in DNA Isolation
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24032156
Abstract: A simplified, fast, and effective production method has been developed for the synthesis of manganese ferrite (MnFe2O4) magnetic nanoparticles (MNPs). In addition to the wide applicability of MnFe2O4 MNPs, this work also reports their application… read more here.
Keywords: dna; application dna; dna isolation; magnetic nanoparticles ... See more keywords