Identification and characterization of novel ligase I inhibitors
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Carcinogenesis"
DOI: 10.1002/mc.22516
Abstract: The terminal step of ligation of single and/or double‐strand breaks during physiological processes such as DNA replication, repair and recombination requires participation of DNA ligases in all mammals. DNA Ligase I has been well characterised… read more here.
Keywords: dna; dna ligase; cancer cells; ligase inhibitors ... See more keywords
1HN, 13C, and 15N backbone resonance assignments of the human DNA ligase 3 DNA-binding domain (residues 257-477)
Sign Up to like & getrecommendations! Published in 2019 at "Biomolecular NMR Assignments"
DOI: 10.1007/s12104-019-09896-9
Abstract: In mammalian cells, the process of DNA ligation is necessary during DNA replication to create an intact “lagging” strand from a series of smaller Okazaki fragments and to repair DNA strand breaks that arise either… read more here.
Keywords: ligase dna; dna; 1hn 13c; dna ligase ... See more keywords
Pol I DNA polymerases stimulate DNA end-joining by Escherichia coli DNA ligase.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2018.01.165
Abstract: Klenow and Klentaq are the large fragment domains of the Pol I DNA polymerases from Escherichia coli and Thermus aquaticus, respectively. Herein, we show that both polymerases can significantly stimulate complementary intermolecular end-joining ligations by… read more here.
Keywords: end joining; dna; coli dna; dna ligase ... See more keywords
DNA ligase and PCNA: Double-ring down to seal a break in DNA.
Sign Up to like & getrecommendations! Published in 2022 at "Structure"
DOI: 10.1016/j.str.2022.02.008
Abstract: In this issue of Structure, Sverzhinsky et al. (2022) report structures of archaeal DNA ligase bound to the proliferating cell nuclear antigen (PCNA) sliding clamp and a nicked DNA substrate. The structures provide snapshots of ligation… read more here.
Keywords: dna; ligase pcna; pcna double; dna ligase ... See more keywords
Cyclization of secondarily structured oligonucleotides to single-stranded rings by using Taq DNA ligase at high temperatures
Sign Up to like & getrecommendations! Published in 2018 at "RSC Advances"
DOI: 10.1039/c8ra02804d
Abstract: Single-stranded DNA rings play important roles in nanoarchitectures, molecular machines, DNA detection, etc. Although T4 DNA ligase has been widely employed to cyclize single-stranded oligonucleotides into rings, the cyclization efficiency is very low when the… read more here.
Keywords: single stranded; dna; taq dna; secondarily structured ... See more keywords
Catalytically inactive DNA ligase IV promotes DNA repair in living cells
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac913
Abstract: Abstract DNA double strand breaks (DSBs) are induced by external genotoxic agents (ionizing radiation or genotoxins) or by internal processes (recombination intermediates in lymphocytes or by replication errors). The DNA ends induced by these genotoxic… read more here.
Keywords: dna repair; inactive lig4; dna; dna ligase ... See more keywords
Structural intermediates of a DNA–ligase complex illuminate the role of the catalytic metal ion and mechanism of phosphodiester bond formation
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkz596
Abstract: Abstract DNA ligases join adjacent 5′ phosphate (5′P) and 3′ hydroxyl (3′OH) termini of double-stranded DNA via a three-step mechanism requiring a nucleotide cofactor and divalent metal ion. Although considerable structural detail is available for… read more here.
Keywords: phosphodiester bond; dna; bond formation; metal ion ... See more keywords
DNA ligase IV deficiency: Immunoglobulin class deficiency depends on the genotype
Sign Up to like & getrecommendations! Published in 2017 at "Pediatric Allergy and Immunology"
DOI: 10.1111/pai.12694
Abstract: DNA ligase IV (LIG4) deficiency (LIG4 syndrome, LS, OMIM 606593) is a very rare autosomal-recessive DNA-repair disorder first reported in 2001(1). In LS, genome instability results in a broad range of Onco-Hemato-Immunological disturbances, including progressive… read more here.
Keywords: dna; ligase deficiency; dna ligase; deficiency ... See more keywords