Single-Molecule Measurements of Motor-Driven Viral DNA Packaging in Bacteriophages Phi29, Lambda, and T4 with Optical Tweezers.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8556-2_20
Abstract: Viral DNA packaging is a required step in the assembly of many dsDNA viruses. A molecular motor fueled by ATP hydrolysis packages the viral genome to near crystalline density inside a preformed prohead shell in… read more here.
Keywords: optical tweezers; dna; motor; dna packaging ... See more keywords
High resolution structure of hexameric herpesvirus DNA-packaging motor elucidates revolving mechanism and ends 20-year fervent debate
Sign Up to like & getrecommendations! Published in 2020 at "Protein & Cell"
DOI: 10.1007/s13238-020-00714-w
Abstract: In this issue of the Protein & Cell Journal, a research team (from Institute of Biophysics, Chinese Academy of Sciences) led by professors Xiangxi Wang and Zihe Rao report the high-resolution structural and physical properties… read more here.
Keywords: dna packaging; herpesvirus; packaging motor; dna ... See more keywords
Nano-channel of viral DNA packaging motor as single pore to differentiate peptides with single amino acid difference.
Sign Up to like & getrecommendations! Published in 2018 at "Biomaterials"
DOI: 10.1016/j.biomaterials.2018.08.005
Abstract: Detection, differentiation, mapping, and sequencing of proteins are important in proteomics for the assessment of cell development such as protein methylation or phosphorylation as well as the diagnosis of diseases including metabolic disorder, mental illness,… read more here.
Keywords: amino; packaging motor; dna; peptides single ... See more keywords
Role of Disulfide Bonds on DNA Packaging Forces in Bull Sperm Chromatin.
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical journal"
DOI: 10.1016/j.bpj.2017.08.050
Abstract: Short arginine-rich proteins called protamines mediate the near crystalline DNA packaging in most vertebrate sperm cells. Protamines are synthesized during spermiogenesis and condense the paternal genome into a transcriptionally inactive state in late-stage spermatids. Protamines… read more here.
Keywords: disulfide bonds; dna packaging; bonds dna; role disulfide ... See more keywords
The many length scales of DNA packaging.
Sign Up to like & getrecommendations! Published in 2019 at "Essays in biochemistry"
DOI: 10.1042/ebc20190040
Abstract: This collection of reviews focuses on the most exciting areas of DNA packaging at the current time. Many of the new discoveries are driven by the development of molecular or imaging techniques, and these are… read more here.
Keywords: scales dna; dna packaging; many length; length scales ... See more keywords
Altering the speed of a DNA packaging motor from bacteriophage T4
Sign Up to like & getrecommendations! Published in 2017 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkx809
Abstract: Abstract The speed at which a molecular motor operates is critically important for the survival of a virus or an organism but very little is known about the underlying mechanisms. Tailed bacteriophage T4 employs one… read more here.
Keywords: bacteriophage; speed; dna packaging; motor ... See more keywords
Structural basis of DNA packaging by a ring-type ATPase from an archetypal viral system
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1101/2022.05.10.491410
Abstract: Many essential cellular processes rely on substrate rotation or translocation by a multi-subunit, ring-type NTPase. A large number of double-stranded DNA viruses, including tailed bacteriophages and herpes viruses, use a homomeric ring ATPase to processively… read more here.
Keywords: system; dna; dna packaging; structural basis ... See more keywords
Mechanical Capsid Maturation Facilitates the Resolution of Conflicting Requirements for Herpesvirus Assembly
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Virology"
DOI: 10.1128/jvi.01831-21
Abstract: The minor capsid protein UL25 plays a critical role in the mechanical maturation of the HSV-1 capsid during virus assembly and is required for stable DNA packaging. We modulated the UL25 capsid interactions by genetically… read more here.
Keywords: capsid; maturation; microscopy; dna packaging ... See more keywords
Full-length human cytomegalovirus terminase pUL89 adopts a two-domain structure specific for DNA packaging
Sign Up to like & getrecommendations! Published in 2019 at "PLoS Pathogens"
DOI: 10.1371/journal.ppat.1008175
Abstract: A key step in replication of human cytomegalovirus (HCMV) in the host cell is the generation and packaging of unit-length genomes into preformed capsids. The enzymes involved in this process are the terminases. The HCMV… read more here.
Keywords: terminase; dna; specific dna; human cytomegalovirus ... See more keywords
Cryo-EM structure and in vitro DNA packaging of a thermophilic virus with supersized T=7 capsids
Sign Up to like & getrecommendations! Published in 2019 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.2210/pdb6i9e/pdb
Abstract: Double-stranded DNA viruses, including bacteriophages and herpesviruses, package their genomes into preformed capsids, using ATP-driven motors. Seeking to advance structural and mechanistic understanding, we established in vitro packaging for a thermostable bacteriophage, P23-45 of Thermus… read more here.
Keywords: dna; capsid; portal protein; dna packaging ... See more keywords
Hinge Region in DNA Packaging Terminase pUL15 of Herpes Simplex Virus: A Potential Allosteric Target for Antiviral Drugs
Sign Up to like & getrecommendations! Published in 2019 at "Biomolecules"
DOI: 10.3390/biom9100603
Abstract: Approximately 80% of adults are infected with a member of the herpesviridae family. Herpesviruses establish life-long latent infections within neurons, which may reactivate into lytic infections due to stress or immune suppression. There are nine… read more here.
Keywords: dna; pul15; packaging; herpes simplex ... See more keywords