DNA-PAINT Super-Resolution Imaging for Characterization of Nucleic Acid Nanostructures.
Sign Up to like & getrecommendations! Published in 2022 at "ChemPlusChem"
DOI: 10.1002/cplu.202200127
Abstract: Numerous nucleic acid nanostructures of unique addressability and programmability have been fabricated for emerging applications. Structural characterization with atomic force microscopy and electron microscopy can provide information on the structural morphology and precision of these… read more here.
Keywords: dna paint; acid nanostructures; microscopy; nucleic acid ... See more keywords
Profiling of Exosomal Biomarkers for Accurate Cancer Identification: Combining DNA-PAINT with Machine- Learning-Based Classification.
Sign Up to like & getrecommendations! Published in 2019 at "Small"
DOI: 10.1002/smll.201901014
Abstract: Exosomes are endosome-derived vesicles enriched in body fluids such as urine, blood, and saliva. So far, they have been recognized as potential biomarkers for cancer diagnostics. However, the present single-variate analysis of exosomes has greatly… read more here.
Keywords: machine learning; analysis; paint machine; cancer ... See more keywords
Real-Time Interfacial Nanothermometry Using DNA-PAINT Microscopy.
Sign Up to like & getrecommendations! Published in 2022 at "Small"
DOI: 10.1002/smll.202201602
Abstract: Biofunctionalized nanoparticles are increasingly used in biomedical applications including sensing, targeted delivery, and hyperthermia. However, laser excitation and associated heating of the nanomaterials may alter the structure and interactions of the conjugated biomolecules. Currently no… read more here.
Keywords: dna paint; interfacial temperature; paint microscopy; microscopy ... See more keywords
DNA-PAINT Super-Resolution Imaging for Nucleic Acid Nanostructures.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6454-3_13
Abstract: Far-field super-resolution fluorescence microscopy has allowed observation of biomolecular and synthetic nanoscale systems with features on the nanometre scale, with chemical specificity and multiplexing capability. DNA-PAINT (DNA-based point accumulation for imaging in nanoscale topography) is… read more here.
Keywords: resolution; super resolution; acid nanostructures; dna paint ... See more keywords
Fluorogenic Probe for Fast 3D Whole-Cell DNA-Paint
Sign Up to like & getrecommendations! Published in 2021 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2020.11.343
Abstract: DNA-PAINT is an increasingly popular super-resolution microscopy method that can acquire high-fidelity images at nanometer resolution. It suffers, however, from high background and very slow imaging speed, both of which can be attributed to the… read more here.
Keywords: probe fast; fluorogenic probe; dna; dna paint ... See more keywords
124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics
Sign Up to like & getrecommendations! Published in 2019 at "Nano Letters"
DOI: 10.1021/acs.nanolett.9b00508
Abstract: Optical super-resolution techniques reach unprecedented spatial resolution down to a few nanometers. However, efficient multiplexing strategies for the simultaneous detection of hundreds of molecular species are still elusive. Here, we introduce an entirely new approach… read more here.
Keywords: resolution; resolution imaging; super resolution; dna paint ... See more keywords
The Role of Nanoscale Distribution of Fibronectin in the Adhesion of Staphylococcus aureus Studied by Protein Patterning and DNA-PAINT
Sign Up to like & getrecommendations! Published in 2022 at "ACS Nano"
DOI: 10.1021/acsnano.2c00630
Abstract: Staphylococcus aureus is a widespread and highly virulent pathogen that can cause superficial and invasive infections. Interactions between S. aureus surface receptors and the extracellular matrix protein fibronectin mediate the bacterial invasion of host cells… read more here.
Keywords: dna paint; adhesion; staphylococcus aureus; fibronectin ... See more keywords
In Situ Super-Resolution Imaging of Telomeres with DNA-PAINT
Sign Up to like & getrecommendations! Published in 2022 at "ACS Omega"
DOI: 10.1021/acsomega.2c05752
Abstract: Telomeres are located at the ends of chromosomes and play an important role in maintaining the integrity of chromosomes and controlling the cycle of cell division. Studies have shown that abnormal telomere length may lead… read more here.
Keywords: dna paint; imaging telomeres; paint; super resolution ... See more keywords
Precision and Accuracy of Receptor Quantification on Synthetic and Biological Surfaces Using DNA-PAINT
Sign Up to like & getrecommendations! Published in 2023 at "ACS Sensors"
DOI: 10.1021/acssensors.2c01736
Abstract: Characterization of the number and distribution of biological molecules on 2D surfaces is of foremost importance in biology and biomedicine. Synthetic surfaces bearing recognition motifs are a cornerstone of biosensors, while receptors on the cell… read more here.
Keywords: dna paint; quantification; accuracy; receptor quantification ... See more keywords
Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-020-20686-z
Abstract: DNA-PAINT is a versatile optical super-resolution technique relying on the transient binding of fluorescent DNA ‘imagers’ to target epitopes. Its performance in biological samples is often constrained by strong background signals and non-specific binding events,… read more here.
Keywords: non specific; paint; dna paint; repeat dna ... See more keywords
Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging
Sign Up to like & getrecommendations! Published in 2018 at "Nature methods"
DOI: 10.1038/s41592-018-0105-0
Abstract: Although current implementations of super-resolution microscopy are technically approaching true molecular-scale resolution, this has not translated to imaging of biological specimens, because of the large size of conventional affinity reagents. Here we introduce slow off-rate… read more here.
Keywords: resolution; modified aptamers; dna paint; microscopy ... See more keywords