Target-Activated DNA Polymerase Activity for Sensitive RNase H Activity Assay.
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DOI: 10.1002/biot.201800645
Abstract: Herein, the ribonuclease H (RNase H) activity assay based on the target-activated DNA polymerase activity is described. In this method, a detection probe composed of two functional sequences, a binding site for DNA polymerase and… read more here.
Keywords: rnase activity; rnase; dna polymerase; activity ... See more keywords
A Single Amino Acid Substitution in Therminator DNA Polymerase Increases Incorporation Efficiency of Deoxyxylonucleotides
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DOI: 10.1002/cbic.201800411
Abstract: Deoxyxylonucleic acid (dxNA) is a synthetic polymer that might have potential for heredity and evolution. Because of dxNA's unusual backbone geometry, sequence information stored in it is presumed to be inaccessible to natural nucleic acids… read more here.
Keywords: dna polymerase; incorporation; polymerase; therminator ... See more keywords
Strand Displacement and Unwinding Assays to Study the Concerted Action of the DNA Polymerase and SSB During Phi29 TP-DNA Replication.
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DOI: 10.1007/978-1-0716-1290-3_22
Abstract: The Bacillus subtilis phage Phi29 has a linear double-stranded DNA with a terminal protein (TP) covalently linked to each 5' end (TP-DNA). Phi29 single-stranded DNA-binding protein (SSB) is encoded by the viral gene 5 and… read more here.
Keywords: phi29 dna; phi29; dna; ssb ... See more keywords
A novel approach for high-level expression and purification of GST-fused highly thermostable Taq DNA polymerase in Escherichia coli
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DOI: 10.1007/s00203-020-01860-9
Abstract: Polymerases are enzymes that synthesize long chains or polymers of nucleic acids including DNA or RNA from nucleotides. They assemble nucleic acids by copying a DNA or RNA template strand using base-pairing interactions. One of… read more here.
Keywords: biology; dna polymerase; taq dna; dna ... See more keywords
DNA polymerase β deficiency in the p53 null cerebellum leads to medulloblastoma formation.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2018.09.166
Abstract: Defects in DNA damage response or repair mechanisms during neurogenesis result in genomic instability, which is causative for several neural defects. These include brain tumors, particularly medulloblastoma, which occurs in the cerebellum with a high… read more here.
Keywords: polymerase deficiency; dna polymerase; p53 null; p53 double ... See more keywords
Biochemical characterization of a unique DNA polymerase A from the extreme radioresistant organism Deinococcus radiodurans.
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DOI: 10.1016/j.biochi.2021.02.014
Abstract: Deinococcus radiodurans survives extraordinary doses of ionizing radiation and desiccation that cause numerous DNA strand breaks. D. radiodurans DNA polymerase A (DrPolA) is essential for reassembling the shattered genome, while its biochemical property has not… read more here.
Keywords: deinococcus radiodurans; drpola ntd; dna polymerase; dna ... See more keywords
Molecular Dynamics Simulations of DNA Pol β Phosphorylation-Induced Structural Changes
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DOI: 10.1016/j.bpj.2016.11.1051
Abstract: DNA polymerase β is a 39 kDa enzyme that is a major component of Base Excision Repair in human cells. The enzyme comprises two major domains, a 31 kDa domain responsible for the polymerase activity… read more here.
Keywords: structural changes; dna polymerase; phosphorylation induced; polymerase ... See more keywords
Switching between Exonucleolysis and Replication by T7 DNA Polymerase Ensures High Fidelity.
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DOI: 10.1016/j.bpj.2016.12.044
Abstract: DNA polymerase catalyzes the accurate transfer of genetic information from one generation to the next, and thus it is vitally important for replication to be faithful. DNA polymerase fulfills the strict requirements for fidelity by… read more here.
Keywords: high fidelity; replication; dna polymerase; dna ... See more keywords
Alternative splicing at exon 2 results in the loss of the catalytic activity of mouse DNA polymerase iota in vitro.
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DOI: 10.1016/j.dnarep.2017.01.001
Abstract: Y-family DNA polymerase iota (Pol ι) possesses both DNA polymerase and dRP lyase activities and was suggested to be involved in DNA translesion synthesis and base excision repair in mammals. The 129 strain of mice… read more here.
Keywords: dna; dna polymerase; polymerase iota; pol ... See more keywords
Replicative DNA polymerase defects in human cancers: Consequences, mechanisms, and implications for therapy.
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DOI: 10.1016/j.dnarep.2017.06.003
Abstract: The fidelity of DNA replication relies on three error avoidance mechanisms acting in series: nucleotide selectivity of replicative DNA polymerases, exonucleolytic proofreading, and post-replicative DNA mismatch repair (MMR). MMR defects are well known to be… read more here.
Keywords: human cancers; dna; dna polymerase; polymerase ... See more keywords
Epigenetically modified N6-methyladenine inhibits DNA replication by human DNA polymerase η.
Sign Up to like & getrecommendations! Published in 2019 at "DNA repair"
DOI: 10.1016/j.dnarep.2019.03.015
Abstract: N6-methyladenine (6mA), as a newly reported epigenetic marker, plays significant roles in regulation of various biological processes in eukaryotes. However, the effect of 6mA on human DNA replication remain elusive. In this work, we used… read more here.
Keywords: dna; human dna; dna replication; dna polymerase ... See more keywords