Hypersensitivity of mouse embryonic fibroblast cells defective for DNA polymerases η, ι and κ to various genotoxic compounds: Its potential for application in chemical genotoxic screening.
Sign Up to like & getrecommendations! Published in 2018 at "DNA repair"
DOI: 10.1016/j.dnarep.2017.11.006
Abstract: Genotoxic agents cause modifications of genomic DNA, such as alkylation, oxidation, bulky adduct formation, and strand breaks, which potentially induce mutations and changes to the structure or number of genes. Majority of point mutations are… read more here.
Keywords: dna; tko cells; dna polymerases; mouse embryonic ... See more keywords
Cooperation between non-essential DNA polymerases contributes to genome stability in Saccharomyces cerevisiae.
Sign Up to like & getrecommendations! Published in 2019 at "DNA repair"
DOI: 10.1016/j.dnarep.2019.02.004
Abstract: DNA polymerases influence genome stability through their involvement in DNA replication, response to DNA damage, and DNA repair processes. Saccharomyces cerevisiae possess four non-essential DNA polymerases, Pol λ, Pol η, Pol ζ, and Rev1, which… read more here.
Keywords: genome stability; dna; non essential; dna polymerases ... See more keywords
Rad5 Recruits Error-Prone DNA Polymerases for Mutagenic Repair of ssDNA Gaps on Undamaged Templates.
Sign Up to like & getrecommendations! Published in 2019 at "Molecular cell"
DOI: 10.1016/j.molcel.2019.01.001
Abstract: Post-replication repair (PRR) allows tolerance of chemical- and UV-induced DNA base lesions in both an error-free and an error-prone manner. In classical PRR, PCNA monoubiquitination recruits translesion synthesis (TLS) DNA polymerases that can replicate through… read more here.
Keywords: dna; dna polymerases; error prone; ssdna gaps ... See more keywords
Processive Activity of Replicative DNA Polymerases in the Replisome of Live Eukaryotic Cells.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular cell"
DOI: 10.1016/j.molcel.2020.08.014
Abstract: DNA replication is carried out by a multi-protein machine called the replisome. In Saccharomyces cerevisiae, the replisome is composed of over 30 different proteins arranged into multiple subassemblies, each performing distinct activities. Synchrony of these… read more here.
Keywords: dna; dna polymerases; activity replicative; processive activity ... See more keywords
Oligoribonucleotide-Mediated Blockade of DNA Extension by Taq DNA Polymerases Increases Specificity and Sensitivity for Detecting Single-Nucleotide Differences.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c05186
Abstract: Blocking PCR is a method that inhibits amplification of DNA possessing a nucleotide sequence complementary to that of a blocker; the method can be used to suppress amplification of target wild-type DNA while amplifying mutated… read more here.
Keywords: dna; extension; taq dna; dna polymerases ... See more keywords
Accurate and efficient one-pot reverse transcription and amplification of 2' fluoro-modified nucleic acids by commercial DNA polymerases.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemistry"
DOI: 10.1021/acs.biochem.0c00494
Abstract: DNA is a foundational tool in biotechnology and synthetic biology but is limited by sensitivity to DNA-modifying enzymes. Recently, researchers have identified DNA polymerases that can enzymatically synthesize long oligonucleotides of modified-DNA (M-DNA) that are… read more here.
Keywords: reverse transcribe; dna polymerases; one pot; dna ... See more keywords
Epigenetic Pyrimidine Nucleotides in Competition with Natural dNTPs as Substrates for Diverse DNA Polymerases
Sign Up to like & getrecommendations! Published in 2022 at "ACS Chemical Biology"
DOI: 10.1021/acschembio.2c00342
Abstract: Five 2′-deoxyribonucleoside triphosphates (dNTPs) derived from epigenetic pyrimidines (5-methylcytosine, 5-hydroxymethylcytosine, 5-formylcytosine, 5-hydroxymethyluracil, and 5-formyluracil) were prepared and systematically studied as substrates for nine DNA polymerases in competition with natural dNTPs by primer extension experiments. The… read more here.
Keywords: natural dntps; dna polymerases; dna; competition natural ... See more keywords
Next-Generation Sequencing-Based Analysis of the Roles of DNA Polymerases ν and θ in the Replicative Bypass of 8-Oxo-7,8-dihydroguanine in Human Cells.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00415
Abstract: DNA polymerase (Pol) ν and Pol θ are two specialized A-family DNA polymerases that function in the translesion synthesis of certain DNA lesions. However, the biological functions of human Pols ν and θ in cellular… read more here.
Keywords: dna; human cells; dna polymerases; replicative bypass ... See more keywords
TT(N)mGCCTC inhibits archaeal family B DNA polymerases
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-20127-4
Abstract: The proofreading activity of the archaeal family B DNA polymerases enables PCR with high fidelity. However, thermostable proofreading DNA polymerases occasionally failed to amplify target fragment that could be amplified by Taq DNA polymerase. We… read more here.
Keywords: family dna; dna; dna polymerases; archaeal family ... See more keywords
An array of basic residues is essential for the nucleolytic activity of the PHP domain of bacterial/archaeal PolX DNA polymerases
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-46349-8
Abstract: Bacterial/archaeal family X DNA polymerases (PolXs) have a C-terminal PHP domain with an active site formed by nine histidines and aspartates that catalyzes 3′-5′ exonuclease, AP-endonuclease, 3′-phosphodiesterase and 3′-phosphatase activities. Multiple sequence alignments have allowed… read more here.
Keywords: bacterial archaeal; domain bacterial; dna polymerases; php domain ... See more keywords
Translesion DNA polymerases in eukaryotes: what makes them tick?
Sign Up to like & getrecommendations! Published in 2017 at "Critical Reviews in Biochemistry and Molecular Biology"
DOI: 10.1080/10409238.2017.1291576
Abstract: Abstract Life as we know it, simply would not exist without DNA replication. All living organisms utilize a complex machinery to duplicate their genomes and the central role in this machinery belongs to replicative DNA… read more here.
Keywords: dna; eukaryotes makes; dna polymerases; polymerases eukaryotes ... See more keywords