High-Resolution Characterization of DNA/Protein Complexes in Living Bacteria.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8675-0_6
Abstract: The occurrence of DNA looping is ubiquitous. This process plays a well-documented role in the regulation of prokaryotic gene expression, such as the Escherichia coli lactose (lac) operon. Here, we present two complementary methods for… read more here.
Keywords: protein; high resolution; dna; dna protein ... See more keywords
Simultaneous detection and quantification of DNA and protein biomarkers in spectrum of cardiovascular diseases in a microfluidic microbead chip
Sign Up to like & getrecommendations! Published in 2019 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-019-02199-x
Abstract: The rapid and simultaneous detection of DNA and protein biomarkers is necessary to detect the outbreak of a disease or to monitor a disease. For example, cardiovascular diseases are a major cause of adult mortality… read more here.
Keywords: detection; dna; dna protein; simultaneous detection ... See more keywords
An Improved Method for Identifying Specific DNA-Protein-Binding Sites In Vitro
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Biotechnology"
DOI: 10.1007/s12033-017-9993-y
Abstract: Binding of proteins to specific DNA sequences is essential for a variety of cellular processes such as DNA replication, transcription and responses to external stimuli. Chromatin immunoprecipitation is widely used for determining intracellular DNA fragments… read more here.
Keywords: protein; protein binding; dna; method identifying ... See more keywords
DeepSite: bidirectional LSTM and CNN models for predicting DNA–protein binding
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Machine Learning and Cybernetics"
DOI: 10.1007/s13042-019-00990-x
Abstract: Transcription factors are cis -regulatory molecules that bind to specific sub-regions of DNA promoters and initiate transcription, the process that regulates the conversion of genetic information from DNA to RNA. Several computational methods have been… read more here.
Keywords: protein binding; dna protein; binding sites; cnn ... See more keywords
5-Formyluracil targeted biochemical reactions with proteins inhibit DNA replication, induce mutations and interference gene expression in living cells
Sign Up to like & getrecommendations! Published in 2021 at "Chinese Chemical Letters"
DOI: 10.1016/j.cclet.2021.02.036
Abstract: Abstract Covalent DNA–protein cross-links are toxic DNA lesions that interfere with essential biological processes, which can cause serious biological consequences, such as genomic instability and protein misexpression. 5-Formyluracil (5fU) as an important modification in DNA,… read more here.
Keywords: gene expression; dna protein; dna; protein ... See more keywords
The ARK Assay Is a Sensitive and Versatile Method for the Global Detection of DNA-Protein Crosslinks
Sign Up to like & getrecommendations! Published in 2020 at "Cell reports"
DOI: 10.1016/j.celrep.2019.12.067
Abstract: SUMMARY DNA-protein crosslinks (DPCs) are a frequent form of DNA lesion and are strongly inhibitive in diverse DNA transactions. Despite recent developments, the biochemical detection of DPCs remains a limiting factor for the in-depth mechanistic… read more here.
Keywords: protein crosslinks; detection; dna; sensitive versatile ... See more keywords
Wss1 Promotes Replication Stress Tolerance by Degrading Histones.
Sign Up to like & getrecommendations! Published in 2020 at "Cell reports"
DOI: 10.1016/j.celrep.2020.02.018
Abstract: Timely completion of DNA replication is central to accurate cell division and to the maintenance of genomic stability. However, certain DNA-protein interactions can physically impede DNA replication fork progression. Cells remove or bypass these physical… read more here.
Keywords: dna; tolerance; dna protein; replication stress ... See more keywords
DNA-protein cross-links: Formidable challenges to maintaining genome integrity.
Sign Up to like & getrecommendations! Published in 2018 at "DNA repair"
DOI: 10.1016/j.dnarep.2018.08.024
Abstract: DNA is associated with proteins that are involved in its folding and transaction processes. When cells are exposed to chemical cross-linking agents or free radical-generating ionizing radiation, DNA-associated proteins are covalently trapped within the DNA… read more here.
Keywords: cross links; dpcs; dna; protein cross ... See more keywords
Immunogenic efficacy of DNA and protein-based vaccine from a chimeric gene consisting OmpW, TcpA and CtxB, of Vibrio cholerae.
Sign Up to like & getrecommendations! Published in 2022 at "Immunobiology"
DOI: 10.1016/j.imbio.2022.152190
Abstract: Vibrio cholerae is one of the major causes of morbidity and mortality in developing countries. CtxB, responsible for toxin binding to eukaryotic cells, TcpA, involved in bacterial colonization, and OmpW, the highly conserved extracellular protein,… read more here.
Keywords: vaccine; protein; dna protein; vibrio cholerae ... See more keywords
Quantification of a Novel DNA-Protein Cross-Link Product Formed by Reacting Apurinic/Apyrimidinic Sites in DNA with Cysteine Residues in Protein by Liquid Chromatography-Tandem Mass Spectrometry Coupled with the Stable Isotope-Dilution Method.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b04306
Abstract: Emerging evidence suggests that cross-links formed by reacting DNA lesions with proteins may play a significant role in the pathophysiology of human cancer and degenerative diseases. The goal of this study was to develop a… read more here.
Keywords: protein cross; dna protein; method; cross ... See more keywords
Primary Amine-Clustered DNA Aptamer for DNA-Protein Conjugation Catalyzed by Microbial Transglutaminase.
Sign Up to like & getrecommendations! Published in 2017 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.7b00594
Abstract: DNA-protein conjugates are promising biomolecules for use in areas ranging from therapeutics to analysis because of the dual functionalities of DNA and protein. Conjugation requires site-specific and efficient covalent bond formation without impairing the activity… read more here.
Keywords: dna aptamer; primary amine; dna protein; dna ... See more keywords