Refined protocols of tamoxifen injection for inducible DNA recombination in mouse astroglia
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-24085-9
Abstract: Inducible DNA recombination of floxed alleles in vivo by liver metabolites of tamoxifen (TAM) is an important tool to study gene functions. Here, we describe protocols for optimal DNA recombination in astrocytes, based on the… read more here.
Keywords: refined protocols; injection; inducible dna; recombination ... See more keywords
Structure-specific DNA recombination sites: Design, validation, and machine learning–based refinement
Sign Up to like & getrecommendations! Published in 2020 at "Science Advances"
DOI: 10.1126/sciadv.aay2922
Abstract: Synthetic attC sites can be embedded into virtually any sequence and constitute a structure-specific DNA recombination system. Recombination systems are widely used as bioengineering tools, but their sites have to be highly similar to a… read more here.
Keywords: dna; structure specific; specific dna; recombination ... See more keywords