LAUSR

DNA rehybridization drives product release from Cas9 ribonucleoprotein to enable multiple-turnover cleavage

Sign Up to like & get
recommendations!
2 Published in 2023 at "Nucleic Acids Research"

DOI: 10.1093/nar/gkad233

Abstract: Abstract The RNA-guided Cas9 endonuclease from Staphylococcus aureus (SauCas9) can catalyze multiple-turnover reactions whereas Cas9 from Streptococcus pyogenes (SpyCas9) is a single-turnover enzyme. Here we dissect the mechanism of multiple-turnover catalysis by SauCas9 and elucidate… read more here.

Keywords: multiple turnover; product; turnover; dna rehybridization ... See more keywords