Programmable Tetrahedral DNA‐RNA Nanocages Woven with Stimuli‐Responsive siRNA for Enhancing Therapeutic Efficacy of Multidrug‐Resistant Tumors
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DOI: 10.1002/advs.202404112
Abstract: Multidrug resistance (MDR) is a major obstacle limiting the effectiveness of chemotherapy against cancer. The combination strategy of chemotherapeutic agents and siRNA targeting drug efflux has emerged as an effective cancer treatment to overcome MDR.… read more here.
Keywords: dna rna; programmable tetrahedral; tetrahedral dna; sirna ... See more keywords
Vector‐Free Deep Tissue Targeting of DNA/RNA Therapeutics via Single Capacitive Discharge Conductivity‐Clamped Gene Electrotransfer (Adv. Sci. 3/2025)
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DOI: 10.1002/advs.202570016
Abstract: DNA/RNA Therapeutics Single pulse electrotransfer of ‘naked’ DNA / mRNA via electric field focusing for deep tissue and vaccine therapeutics. In article 2406545, Jeremy L. Pinyon, Georg von Jonquieres, Gary D. Housley, and co‐workers describe… read more here.
Keywords: dna rna; deep tissue; electrotransfer; rna therapeutics ... See more keywords
Optimization of pre‐analytical and analytical steps for DNA and RNA analysis of fresh cytology samples
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DOI: 10.1002/cam4.4728
Abstract: Different cytology preparations can be used for molecular diagnostics, however the influence of pre‐analytical and analytical steps on the results are not yet well defined. We aimed to determine optimal steps for efficient extraction of… read more here.
Keywords: analytical analytical; optimization pre; analytical steps; pre analytical ... See more keywords
Spectroscopic and in vitro Investigations of Fe2+/α‐Ketoglutarate‐Dependent Enzymes Involved in Nucleic Acid Repair and Modification
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DOI: 10.1002/cbic.202100605
Abstract: The activation of molecular oxygen for the highly selective functionalization and repair of DNA and RNA nucleobases is achieved by α‐ketoglutarate (α‐KG)/iron‐dependent dioxygenases. Of special interest are the human homologues AlkBH of Escherichia coli EcAlkB… read more here.
Keywords: enzymes involved; spectroscopic vitro; repair modification; dna rna ... See more keywords
Preanalytic specimen triage: Smears, cell blocks, cytospin preparations, transport media, and cytobanking
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DOI: 10.1002/cncy.21850
Abstract: With increasing requests for the evaluation of prognostic and predictive molecular biomarkers, great attention must be paid to the preanalytical issues regarding sample quality and DNA/RNA yield from all different types of cytological preparations. The… read more here.
Keywords: specimen triage; dna rna; preanalytic specimen; cytology ... See more keywords
Cancer gene analysis of liquid-based cytology specimens using next-generation sequencing: A technical report of bimodal DNA- and RNA-based panel application.
Sign Up to like & getrecommendations! Published in 2023 at "Diagnostic cytopathology"
DOI: 10.1002/dc.25149
Abstract: BACKGROUND As liquid-based cytology (LBC) specimens harbor high-quality DNA, genomic analysis using LBC specimens is beneficial for integrative diagnosis. This study aimed to clarify the feasibility of LBC specimens for a bimodal application of DNA-… read more here.
Keywords: gene; lbc specimens; dna rna; cytology ... See more keywords
Dysbiosis of the stool DNA and RNA virome in Crohn's disease
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DOI: 10.1002/jmv.28573
Abstract: Pathogenesis of Crohn's disease (CD) relates to gut microbiome dysbiosis. However, less is known about the viral microbiome, consisting of bacteriophages and eukaryotic viruses, in CD. Here, we profiled the stool virome, viral functions, and… read more here.
Keywords: disease; dysbiosis; crohn disease; rna virome ... See more keywords
A Chimeric DNA/RNA Antiparallel Quadruplex with Improved Stability
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DOI: 10.1002/open.202100276
Abstract: Abstract Nucleic acid quadruplexes are proposed to play a role in the regulation of gene expression, are often present in aptamers selected for specific binding functions and have potential applications in medicine and biotechnology. Therefore,… read more here.
Keywords: topology; dna; stability; antiparallel quadruplex ... See more keywords
Protein–DNA/RNA interactions: Machine intelligence tools and approaches in the era of artificial intelligence and big data
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DOI: 10.1002/pmic.202100197
Abstract: With the development of artificial intelligence (AI) technologies and the availability of large amounts of biological data, computational methods for proteomics have undergone a developmental process from traditional machine learning to deep learning. This review… read more here.
Keywords: intelligence; machine; artificial intelligence; rna interactions ... See more keywords
Simultaneous DNA, RNA, and Protein Analysis from Single Cells Using a High-Throughput Microfluidic Workflow for Resolution of Genotype-to-Phenotype Modalities.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1771-7_17
Abstract: Understanding the genomic landscape of cancer in single cells can be valuable for the characterization of molecular events that drive evolution of tumorigenesis and fostering progress in identifying druggable regimens for patient treatment scenarios. We… read more here.
Keywords: cells using; dna rna; single cells; simultaneous dna ... See more keywords
Be bold, start cold! cold formalin fixation of colorectal cancer specimens granted superior DNA and RNA quality for downstream molecular analysis
Sign Up to like & getrecommendations! Published in 2024 at "Histochemistry and Cell Biology"
DOI: 10.1007/s00418-024-02326-5
Abstract: The use of cold formalin fixation (CFF; i.e., fixating tissue samples with 4 °C precooled formalin) recently attracted further attention owing to its putative improved ability to preserve nucleic acid compared with standard room temperature… read more here.
Keywords: dna rna; formalin; cold formalin; formalin fixation ... See more keywords