Optimization of pre‐analytical and analytical steps for DNA and RNA analysis of fresh cytology samples
Sign Up to like & getrecommendations! Published in 2022 at "Cancer Medicine"
DOI: 10.1002/cam4.4728
Abstract: Different cytology preparations can be used for molecular diagnostics, however the influence of pre‐analytical and analytical steps on the results are not yet well defined. We aimed to determine optimal steps for efficient extraction of… read more here.
Keywords: analytical analytical; optimization pre; analytical steps; pre analytical ... See more keywords
Spectroscopic and in vitro Investigations of Fe2+/α‐Ketoglutarate‐Dependent Enzymes Involved in Nucleic Acid Repair and Modification
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202100605
Abstract: The activation of molecular oxygen for the highly selective functionalization and repair of DNA and RNA nucleobases is achieved by α‐ketoglutarate (α‐KG)/iron‐dependent dioxygenases. Of special interest are the human homologues AlkBH of Escherichia coli EcAlkB… read more here.
Keywords: enzymes involved; spectroscopic vitro; repair modification; dna rna ... See more keywords
Preanalytic specimen triage: Smears, cell blocks, cytospin preparations, transport media, and cytobanking
Sign Up to like & getrecommendations! Published in 2017 at "Cancer Cytopathology"
DOI: 10.1002/cncy.21850
Abstract: With increasing requests for the evaluation of prognostic and predictive molecular biomarkers, great attention must be paid to the preanalytical issues regarding sample quality and DNA/RNA yield from all different types of cytological preparations. The… read more here.
Keywords: specimen triage; dna rna; preanalytic specimen; cytology ... See more keywords
Cancer gene analysis of liquid-based cytology specimens using next-generation sequencing: A technical report of bimodal DNA- and RNA-based panel application.
Sign Up to like & getrecommendations! Published in 2023 at "Diagnostic cytopathology"
DOI: 10.1002/dc.25149
Abstract: BACKGROUND As liquid-based cytology (LBC) specimens harbor high-quality DNA, genomic analysis using LBC specimens is beneficial for integrative diagnosis. This study aimed to clarify the feasibility of LBC specimens for a bimodal application of DNA-… read more here.
Keywords: gene; lbc specimens; dna rna; cytology ... See more keywords
Dysbiosis of the stool DNA and RNA virome in Crohn's disease
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Medical Virology"
DOI: 10.1002/jmv.28573
Abstract: Pathogenesis of Crohn's disease (CD) relates to gut microbiome dysbiosis. However, less is known about the viral microbiome, consisting of bacteriophages and eukaryotic viruses, in CD. Here, we profiled the stool virome, viral functions, and… read more here.
Keywords: disease; dysbiosis; crohn disease; rna virome ... See more keywords
A Chimeric DNA/RNA Antiparallel Quadruplex with Improved Stability
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DOI: 10.1002/open.202100276
Abstract: Abstract Nucleic acid quadruplexes are proposed to play a role in the regulation of gene expression, are often present in aptamers selected for specific binding functions and have potential applications in medicine and biotechnology. Therefore,… read more here.
Keywords: topology; dna; stability; antiparallel quadruplex ... See more keywords
Protein–DNA/RNA interactions: Machine intelligence tools and approaches in the era of artificial intelligence and big data
Sign Up to like & getrecommendations! Published in 2022 at "PROTEOMICS"
DOI: 10.1002/pmic.202100197
Abstract: With the development of artificial intelligence (AI) technologies and the availability of large amounts of biological data, computational methods for proteomics have undergone a developmental process from traditional machine learning to deep learning. This review… read more here.
Keywords: intelligence; machine; artificial intelligence; rna interactions ... See more keywords
Simultaneous DNA, RNA, and Protein Analysis from Single Cells Using a High-Throughput Microfluidic Workflow for Resolution of Genotype-to-Phenotype Modalities.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1771-7_17
Abstract: Understanding the genomic landscape of cancer in single cells can be valuable for the characterization of molecular events that drive evolution of tumorigenesis and fostering progress in identifying druggable regimens for patient treatment scenarios. We… read more here.
Keywords: cells using; dna rna; single cells; simultaneous dna ... See more keywords
Structural characteristics of cyclopentane-modified peptide nucleic acids from molecular dynamics simulations
Sign Up to like & getrecommendations! Published in 2017 at "Structural Chemistry"
DOI: 10.1007/s11224-017-0970-7
Abstract: A PNA molecule is a DNA strand where the sugar-phosphate backbone has been replaced by a structurally homomorphous pseudopeptide chain consisting of N (2-amino-ethyl)-glycine units. PNA binds strongly to both DNA and RNA. However, an… read more here.
Keywords: nucleic acids; dna; peptide nucleic; pna ... See more keywords
Mapping the epigenetic modifications of DNA and RNA
Sign Up to like & getrecommendations! Published in 2020 at "Protein & Cell"
DOI: 10.1007/s13238-020-00733-7
Abstract: Over 17 and 160 types of chemical modifications have been identified in DNA and RNA, respectively. The interest in understanding the various biological functions of DNA and RNA modifications has lead to the cutting-edged fields… read more here.
Keywords: modifications dna; mapping epigenetic; dna rna; epigenetic modifications ... See more keywords
Ion Mobility-Mass Spectrometry Reveals Details of Formation and Structure for GAA·TCC DNA and RNA Triplexes
Sign Up to like & getrecommendations! Published in 2018 at "Journal of The American Society for Mass Spectrometry"
DOI: 10.1007/s13361-018-2077-9
Abstract: AbstractDNA and RNA triplexes are thought to play key roles in a range of cellular processes such as gene regulation and epigenetic remodeling and have been implicated in human disease such as Friedreich’s ataxia. In… read more here.
Keywords: mass spectrometry; dna; rna; rna triplexes ... See more keywords