Design of customizable long linear DNA substrates with controlled end modifications for single-molecule studies.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2019.113541
Abstract: Many strategies have been developed to manipulate DNA molecules and investigate protein-DNA interactions with single-molecule resolution. Often, these require long DNA molecules with a length of several 10s of kb that are chemically modified at… read more here.
Keywords: dna molecules; single molecule; dna; dna substrates ... See more keywords
HMGB1 Stimulates Activity of Polymerase β on Nucleosome Substrates.
Sign Up to like & getrecommendations! Published in 2017 at "Biochemistry"
DOI: 10.1021/acs.biochem.6b00569
Abstract: The process of base excision repair (BER) recognizes and repairs small lesions or inappropriate bases on DNA through either a short-patch or long-patch pathway. The enzymes involved in BER have been well-characterized on DNA substrates,… read more here.
Keywords: dna; dna substrates; polymerase; pol ... See more keywords
Site-Specific Covalent Labeling of DNA Substrates by an RNA Transglycosylase
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.3c00861
Abstract: Bacterial tRNA guanine transglycosylases (TGTs) catalyze the exchange of guanine for the 7-deazaguanine queuine precursor, prequeuosine1 (preQ1). While the native nucleic acid substrate for bacterial TGTs is the anticodon loop of queuine-cognate tRNAs, the minimum… read more here.
Keywords: specific covalent; dna; rna; dna substrates ... See more keywords
PAM-less conditional DNA substrates leverage trans-cleavage of CRISPR-Cas12a for versatile live-cell biosensing
Sign Up to like & getrecommendations! Published in 2022 at "Chemical Science"
DOI: 10.1039/d1sc05558e
Abstract: The CRISPR-Cas system has been repurposed as a powerful live-cell imaging tool, but its utility is limited to genomic loci and mRNA imaging in living cells. Here, we demonstrated the potential of the CRISPR-Cas system… read more here.
Keywords: system; crispr cas12a; cell biosensing; dna substrates ... See more keywords
Structural and optical variation of pseudoisocyanine aggregates nucleated on DNA substrates
Sign Up to like & getrecommendations! Published in 2023 at "Methods and Applications in Fluorescence"
DOI: 10.1088/2050-6120/acb2b4
Abstract: Coherently coupled pseudoisocyanine (PIC) dye aggregates have demonstrated the ability to delocalize electronic excitations and ultimately migrate excitons with much higher efficiency than similar designs where excitations are isolated to individual chromophores. Here, we report… read more here.
Keywords: pseudoisocyanine; dna; variation; dna substrates ... See more keywords