Rad53 arrests leading and lagging strand DNA synthesis via distinct mechanisms in response to DNA replication stress
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DOI: 10.1002/bies.202200061
Abstract: DNA replication stress threatens ordinary DNA synthesis. The evolutionarily conserved DNA replication stress response pathway involves sensor kinase Mec1/ATR, adaptor protein Mrc1/Claspin, and effector kinase Rad53/Chk1, which spurs a host of changes to stabilize replication… read more here.
Keywords: replication stress; dna synthesis; replication; dna ... See more keywords
Proteomic Analysis of DNA Synthesis on a Structured DNA Template in Human Cellular Extracts: Interplay Between NHEJ and Replication‐Associated Proteins
Sign Up to like & getrecommendations! Published in 2020 at "PROTEOMICS"
DOI: 10.1002/pmic.201900184
Abstract: It is established that short inverted repeats trigger base substitution mutagenesis in human cells. However, how the replication machinery deals with structured DNA is unknown. It has been previously reported that in human cell‐free extracts,… read more here.
Keywords: dna synthesis; dna; template; dna template ... See more keywords
Artificial Nucleosides as Diagnostic Probes to Measure Translesion DNA Synthesis.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9216-4_15
Abstract: The misreplication of damaged DNA, a biological process termed translesion DNA synthesis (TLS), produces a large number of adverse effects on human health. This chapter describes the application of an artificial nucleoside/nucleotide system that functions… read more here.
Keywords: translesion dna; dna synthesis; chemistry; dna ... See more keywords
Sgs1 helicase is required for efficient PCNA monoubiquitination and translesion DNA synthesis in Saccharomyces cerevisiae
Sign Up to like & getrecommendations! Published in 2017 at "Current Genetics"
DOI: 10.1007/s00294-017-0753-0
Abstract: DNA-damage tolerance (DDT) is employed by eukaryotes to deal with replication blocks on the template strand, and is divided into two parallel pathways that are activated by sequential ubiquitination of proliferating cell nuclear antigen (PCNA)… read more here.
Keywords: dna synthesis; pcna monoubiquitination; dna; sgs1 ... See more keywords
Profile of anti‐herpetic action of ASP2151 (amenamevir) as a helicase‐primase inhibitor
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DOI: 10.1016/j.antiviral.2016.12.008
Abstract: &NA; The antiherpetic drugs acyclovir (ACV, valaciclovir) and penciclovir (famciclovir) are phosphorylated by viral thymidine kinase and terminate DNA synthesis. ASP2151 (amenamevir) and foscavir (PFA) directly inhibit viral helicase‐primase and DNA polymerase, respectively, and inhibit… read more here.
Keywords: dna synthesis; dna; asp2151; viral dna ... See more keywords
Distinct dual antiviral mechanism that enhances hepatitis B virus mutagenesis and reduces viral DNA synthesis.
Sign Up to like & getrecommendations! Published in 2019 at "Antiviral research"
DOI: 10.1016/j.antiviral.2019.104540
Abstract: Reverse transcriptase (RT) is an essential enzyme for the replication of retroviruses and hepadnaviruses. Current therapies do not eliminate the intracellular viral replication intermediate termed covalently closed circular (ccc) DNA, which has enhanced interest in… read more here.
Keywords: dna synthesis; mutagenesis; dna; aza ... See more keywords
DNA replication is highly resilient and persistent under the challenge of mild replication stress.
Sign Up to like & getrecommendations! Published in 2022 at "Cell reports"
DOI: 10.1016/j.celrep.2022.110701
Abstract: Mitotic DNA synthesis (MiDAS) has been proposed to restart DNA synthesis during mitosis because of replication fork stalling in late interphase caused by mild replication stress (RS). Contrary to this proposal, we find that cells… read more here.
Keywords: mild replication; dna; dna synthesis; replication ... See more keywords
Mini review: Enzyme-based DNA synthesis and selective retrieval for data storage
Sign Up to like & getrecommendations! Published in 2021 at "Computational and Structural Biotechnology Journal"
DOI: 10.1016/j.csbj.2021.04.057
Abstract: The market for using and storing digital data is growing, with DNA synthesis emerging as an efficient way to store massive amounts of data. Storing information in DNA mainly consists of two steps: data writing… read more here.
Keywords: dna synthesis; dna; enzyme based; based dna ... See more keywords
Aedes aegypti antiviral adaptive response against DENV‐2
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DOI: 10.1016/j.dci.2018.01.022
Abstract: &NA; Priming is the conceptual term defining memory phenomenon in innate immune response. Numerous examples of enhanced secondary immune response have been described in diverse taxa of invertebrates; which naturally lacks memory response. In mosquitoes,… read more here.
Keywords: dna synthesis; aedes aegypti; response; denv ... See more keywords
Single-Atom-Directed Inhibition of De Novo DNA Synthesis in Isothermal Amplifications.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c03489
Abstract: The template-dependent DNA synthesis, with DNA polymerases, templates, and primers, is essential for disease detection, molecular biology, and biotechnology. However, DNA polymerases can also initiate de novo DNA synthesis without templates and primers, forming byproduct… read more here.
Keywords: dna; atom; novo dna; synthesis ... See more keywords
Synthesis of Substituted Cy5 Phosphoramidite Derivatives and Their Incorporation into Oligonucleotides Using Automated DNA Synthesis
Sign Up to like & getrecommendations! Published in 2022 at "ACS Omega"
DOI: 10.1021/acsomega.1c06921
Abstract: Cyanine dyes represent a family of organic fluorophores with widespread utility in biological-based applications ranging from real-time PCR probes to protein labeling. One burgeoning use currently being explored with indodicarbocyanine (Cy5) in particular is that… read more here.
Keywords: dna; synthesis; dna synthesis; incorporation ... See more keywords