"Just Add Small Molecules" Cell-Free Protein Synthesis: Combining DNA Template and Cell Extract Preparation into a Single Fermentation.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology progress"
DOI: 10.1002/btpr.3332
Abstract: Cell-free protein synthesis (CFPS) is a versatile biotechnology platform enabling a broad range of applications including clinical diagnostics, large-scale production of officinal therapeutics, small-scale on-demand production of personal magistral therapeutics, and exploratory research. The shelf… read more here.
Keywords: extract preparation; cell; dna; preparation ... See more keywords
Proteomic Analysis of DNA Synthesis on a Structured DNA Template in Human Cellular Extracts: Interplay Between NHEJ and Replication‐Associated Proteins
Sign Up to like & getrecommendations! Published in 2020 at "PROTEOMICS"
DOI: 10.1002/pmic.201900184
Abstract: It is established that short inverted repeats trigger base substitution mutagenesis in human cells. However, how the replication machinery deals with structured DNA is unknown. It has been previously reported that in human cell‐free extracts,… read more here.
Keywords: dna synthesis; dna; template; dna template ... See more keywords
Bio-molecule templated hydrothermal synthesis of ZnWO4 nanomaterial for high-performance supercapacitor electrode application
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Molecular Structure"
DOI: 10.1016/j.molstruc.2018.12.087
Abstract: Abstract ZnWO4 nanomaterials have been synthesized through DNA template approach with variable hydrothermal reaction time. The affirmation of formation of crystalline ZnWO4 nanomaterials were examined by X- Ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR),… read more here.
Keywords: dna template; performance; znwo4; znwo4 nanomaterials ... See more keywords
Effects of DNA template preparation on variability in cell-free protein production.
Sign Up to like & getrecommendations! Published in 2022 at "Synthetic biology"
DOI: 10.1093/synbio/ysac015
Abstract: DNA templates for protein production remain an unexplored source of variability in the performance of cell-free expression (CFE) systems. To characterize this variability, we investigated the effects of two common DNA extraction methodologies, a postprocessing… read more here.
Keywords: protein production; variability; dna; dna template ... See more keywords