DNA-Unwinding Dynamics of Escherichia coli UvrD Lacking the C-terminal 40 Amino Acids.
Sign Up to like & getrecommendations! Published in 2020 at "Biophysical journal"
DOI: 10.1016/j.bpj.2020.02.014
Abstract: The E. coli UvrD protein is a nonhexameric DNA helicase that belongs to superfamily I and plays a crucial role in both nucleotide excision repair and methyl-directed mismatch repair. Previous data suggested that wild-type UvrD has… read more here.
Keywords: amino acids; dna; 40c molecules; terminal amino ... See more keywords
A model of DNA unwinding dynamics by the RecBCD complex and its regulation by Chi recognition.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of theoretical biology"
DOI: 10.1016/j.jtbi.2018.04.014
Abstract: The Escherichia coli RecBCD enzyme is a heterotrimeric helicase-nuclease complex responsible for processing of double-stranded DNA breaks for repair by homologous recombination. It is a highly processive, duplex unwinding and degrading motor, with its activities… read more here.
Keywords: chi; model; complex regulation; recbcd complex ... See more keywords
Control of Hexamerization, Assembly, and Excluded Strand Specificity for the Sulfolobus solfataricus MCM Helicase.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemistry"
DOI: 10.1021/acs.biochem.8b00766
Abstract: A growing body of evidence supports a steric exclusion and wrapping model for DNA unwinding in which hexameric helicases interact with the excluded single-stranded DNA (ssDNA) in addition to the encircled strand. Interactions with the… read more here.
Keywords: strand; solfataricus mcm; sulfolobus solfataricus; excluded strand ... See more keywords
The convergence of head-on DNA unwinding forks induces helicase oligomerization and activity transition
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2116462119
Abstract: Significance Bloom syndrome helicase (BLM) is a multifunctional helicase that primarily catalyzes the separation of two single strands of DNA. Here, using a single-molecule optical tweezers approach combined with confocal microscopy, we monitored both the… read more here.
Keywords: convergence head; unwinding forks; dna unwinding; head dna ... See more keywords
Dynamic structural insights into the molecular mechanism of DNA unwinding by the bacteriophage T7 helicase
Sign Up to like & getrecommendations! Published in 2020 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa057
Abstract: Abstract The hexametric T7 helicase (gp4) adopts a spiral lock-washer form and encircles a coil-like DNA (tracking) strand with two nucleotides bound to each subunit. However, the chemo-mechanical coupling mechanism in unwinding has yet to… read more here.
Keywords: gp4; mechanism; dna; helicase ... See more keywords
Human RPA activates BLM’s bidirectional DNA unwinding from a nick
Sign Up to like & getrecommendations! Published in 2020 at "eLife"
DOI: 10.7554/elife.54098
Abstract: BLM is a multifunctional helicase that plays critical roles in maintaining genome stability. It processes distinct DNA substrates, but not nicked DNA, during many steps in DNA replication and repair. However, how BLM prepares itself… read more here.
Keywords: dna; dna unwinding; activates blm; blm bidirectional ... See more keywords