Real-time analysis of initiation regulation systems during the progression of the reconstituted chromosomal replication cycle
Sign Up to like & getrecommendations! Published in 2025 at "Scientific Reports"
DOI: 10.1038/s41598-025-88988-0
Abstract: Chromosome replication in Escherichia coli is primarily regulated at the initiation stage, where the DnaA protein activates replication at the chromosomal origin, oriC. Both DnaA and oriC undergo feedback regulation based on replication progression. Previously,… read more here.
Keywords: replication; initiation; replication cycle; dnaa ... See more keywords
Single-stranded DNA recruitment mechanism in replication origin unwinding by DnaA initiator protein and HU, an evolutionary ubiquitous nucleoid protein.
Sign Up to like & getrecommendations! Published in 2023 at "Nucleic acids research"
DOI: 10.1093/nar/gkad389
Abstract: The Escherichia coli replication origin oriC contains the initiator ATP-DnaA-Oligomerization Region (DOR) and its flanking duplex unwinding element (DUE). In the Left-DOR subregion, ATP-DnaA forms a pentamer by binding to R1, R5M and three other DnaA… read more here.
Keywords: dnaa; r5m; replication origin; due unwinding ... See more keywords
Mapping the Escherichia coli DnaA-binding landscape reveals a preference for binding pairs of closely spaced DNA sites
Sign Up to like & getrecommendations! Published in 2024 at "Microbiology"
DOI: 10.1099/mic.0.001474
Abstract: Abstract DnaA is a widely conserved DNA-binding protein that is essential for the initiation of DNA replication in many bacterial species, including Escherichia coli. Cooperative binding of ATP-bound DnaA to multiple 9mer sites (‘DnaA boxes’)… read more here.
Keywords: coli dnaa; replication; escherichia coli; dnaa ... See more keywords
Lon recognition of the replication initiator DnaA requires a bipartite degron
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Microbiology"
DOI: 10.1111/mmi.14146
Abstract: DnaA initiates chromosome replication in bacteria. In Caulobacter crescentus, the Lon protease degrades DnaA to coordinate replication with nutrient availability and to halt the cell cycle during acute stress. Here, we characterize the mechanism of… read more here.
Keywords: lon; degradation; lon recognition; replication ... See more keywords
The linker domain of the initiator DnaA contributes to its ATP binding and membrane association in E. coli chromosomal replication
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1126/sciadv.abq6657
Abstract: DnaA, the initiator of Escherichia coli chromosomal replication, has in its adenosine triphosphatase (ATPase) domain residues required for adenosine 5′-triphosphate (ATP) binding and membrane attachment. Here, we show that D118Q substitution in the DnaA linker… read more here.
Keywords: dnaa; linker domain; replication; atp binding ... See more keywords
Rapid turnover of DnaA at replication origin regions contributes to initiation control of DNA replication
Sign Up to like & getrecommendations! Published in 2017 at "PLoS Genetics"
DOI: 10.1371/journal.pgen.1006561
Abstract: DnaA is a conserved key regulator of replication initiation in bacteria, and is homologous to ORC proteins in archaea and in eukaryotic cells. The ATPase binds to several high affinity binding sites at the origin… read more here.
Keywords: initiation; origin regions; turnover; time ... See more keywords
Energy Starvation Induces a Cell Cycle Arrest in Escherichia coli by Triggering Degradation of the DnaA Initiator Protein
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2021.629953
Abstract: During steady-state Escherichia coli growth, the amount and activity of the initiator protein, DnaA, controls chromosome replication tightly so that initiation only takes place once per origin in each cell cycle, regardless of growth conditions.… read more here.
Keywords: starvation; escherichia coli; cell cycle; initiator protein ... See more keywords