DNAzyme–aptamer or aptamer–DNAzyme paradigm: Biochemical approach for aflatoxin analysis
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.1563
Abstract: DNAzyme and aptamer conjugations have already been used for sensitive and accurate detection of several molecules. In this study, we tested the relationship between conjugation orientation of DNAzyme and aflatoxin B1 aptamer and their subsequent… read more here.
Keywords: dnazyme; analysis; aptamer dnazyme; dnazyme aptamer ... See more keywords
Label-free DNAzyme for highly sensitive detection of multiple biomolecules in real samples through target-triggered catalytic cleavage reactions with auramine O’s discriminated fluorescence emission
Sign Up to like & getrecommendations! Published in 2022 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-022-04061-z
Abstract: A universal enzyme strand (E-DNA) recyclable L-histidine (L-His), melamine (MA), and cisplatin (CP) biosensor was fabricated on the basis of a target-specific RNA-cleaving DNAzyme with specific auramine O (AuO) dye instead of thioflavin T. In… read more here.
Keywords: cleavage; dnazyme; dna; emission ... See more keywords
Proximity ligation assay induced hairpin to DNAzyme structure switching for entropy-driven amplified detection of thrombin.
Sign Up to like & getrecommendations! Published in 2019 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2019.03.007
Abstract: A proximity ligation assay (PLA) induced hairpin to DNAzyme structure switching strategy has been described for entropy-driven amplified detection of thrombin. The enzyme-strand (E-DNA) and substrate-strand (S-DNA) of DNAzyme are locked in hairpins structure, and… read more here.
Keywords: dnazyme; proximity ligation; entropy driven; structure ... See more keywords
A DNAzyme sensor based on target-catalyzed hairpin assembly for enzyme-free and non-label single nucleotide polymorphism genotyping.
Sign Up to like & getrecommendations! Published in 2017 at "Talanta"
DOI: 10.1016/j.talanta.2017.03.001
Abstract: Single nucleotide polymorphisms (SNPs) are widely existed in human genome and associated with many diseases. Traditional PCR-based methods for SNP genotyping require protein enzyme, precise control of temperature and removal of resultant products, making the… read more here.
Keywords: enzyme free; dnazyme; single nucleotide; free non ... See more keywords
Ratiometric fluorescent detection and imaging of microRNA in living cells with manganese dioxide nanosheet-active DNAzyme.
Sign Up to like & getrecommendations! Published in 2021 at "Talanta"
DOI: 10.1016/j.talanta.2021.122518
Abstract: MicroRNAs (miRNAs) play an important role in multiple biological processes and can be used as biomarkers for clinical disease diagnosis, so their detection is of great importance. Here, manganese dioxide (MnO2) nanosheet acts as carrier… read more here.
Keywords: detection; dnazyme; fluorescent detection; ratiometric fluorescent ... See more keywords
DNAzyme-based sensing probe protected by DNA tetrahedron from nuclease degradation for the detection of lead ions.
Sign Up to like & getrecommendations! Published in 2021 at "Talanta"
DOI: 10.1016/j.talanta.2021.122543
Abstract: Lead poisoning endangers soil, plants and human health due to its toxic effect. It is urgent to develop ideal tool for the in vivo detection of Pb2+.In this study, tetrahedron-based Pb2+-sensitive DNAzyme sensor (TPS) is… read more here.
Keywords: pb2; sensing probe; detection; dnazyme ... See more keywords
Highly Sensitive Biosensing Applications of a Magnetically Immobilizable Covalent G-Quadruplex-Hemin DNAzyme Catalytic System.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c04842
Abstract: G-quadruplex/hemin (G4/hemin) DNAzymes are biosensing systems, but their application remains limited by an overall low activity and a rather high level of unwarranted background reactions. Here, these issues were addressed through the rational design of… read more here.
Keywords: system; dnazyme; hemin; quadruplex hemin ... See more keywords
Construction of an Entropy-Driven Dumbbell-Type DNAzyme Assembly Circuit for Lighting Up Uracil-DNA Glycosylase in Living Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c03223
Abstract: Sensitive monitoring of intracellular uracil-DNA glycosylase (UDG) in living cells is essential to understanding the DNA repair pathways and discovery of anticancer drugs. Herein, we demonstrate the construction of an entropy-driven dumbbell-type DNAzyme assembly circuit… read more here.
Keywords: dna; entropy driven; dnazyme; uracil dna ... See more keywords
Csm6-DNAzyme Tandem Assay for One-Pot and Sensitive Analysis of Lead Pollution and Bioaccumulation in Mice.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c04589
Abstract: Lead contamination in the environment tends to enter the food chain and further into the human body, causing serious health issues. Herein, we proposed a Csm6-DNAzyme tandem assay (termed cDNAzyme) using CRISPR/Cas III-A Csm6 and… read more here.
Keywords: csm6 dnazyme; dnazyme; dnazyme tandem; csm6 ... See more keywords
MicroRNA Imaging Encounters Rolling Circle Amplification: Intracellular Na+-Fueled Linear Programmable DNAzyme Nanostructure.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.3c00293
Abstract: DNAzyme motors are widely used for the sensitive detection of intracellular miRNAs due to their excellent signal response. Generally, the addition of exogenous mental ions to DNAzyme motors is crucial for the efficient operation of… read more here.
Keywords: dnazyme nanostructure; dnazyme; rolling circle; linear programmable ... See more keywords
Dual-Analyte Chronopotentiometric Aptasensing Platform Based on a G-Quadruplex/Hemin DNAzyme and Logic Gate Operations.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b05971
Abstract: Conventional potentiometric ion sensors that rely on a specific ion carrier in a polymeric membrane can hardly achieve multianalyte detection. Inspired by the remarkable ability of built-in logic gate sensors for multianalyte detection, herein we… read more here.
Keywords: dnazyme; based quadruplex; hemin dnazyme; logic gate ... See more keywords