Single domain antibodies for the knockdown of cytosolic and nuclear proteins.
Sign Up to like & getrecommendations! Published in 2017 at "Protein Science"
DOI: 10.1002/pro.3154
Abstract: Single domain antibodies (sdAbs) from camels or sharks comprise only the variable heavy chain domain. Human sdAbs comprise the variable domain of the heavy chain (VH) or light chain (VL) and can be selected from… read more here.
Keywords: sdabs; single domain; domain antibodies; cytosolic nuclear ... See more keywords
Optimisation of human VH domain antibodies specific to Mycobacterium tuberculosis heat shock protein (HSP16.3)
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Computer-Aided Molecular Design"
DOI: 10.1007/s10822-019-00186-z
Abstract: Mycobacterium tuberculosis (Mtb) 16.3 kDa heat shock protein 16.3 (HSP16.3) is a latency-associated antigen that can be targeted for latent tuberculosis (TB) diagnostic and therapeutic development. We have previously developed human VH domain antibodies (dAbs; clone… read more here.
Keywords: hsp16; domain antibodies; mycobacterium tuberculosis; heat shock ... See more keywords
Single domain antibodies in the development of immunosensors for diagnostics.
Sign Up to like & getrecommendations! Published in 2020 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2020.10.031
Abstract: Scientific advances in nanotechnology and nanoscience have enabled stability optimization and signal amplification in immunoassays by taking advantage of unique properties of nanomaterials. Biosensors based on antibodies and their fragments, also called immunosensors, are compact… read more here.
Keywords: domain antibodies; antibodies development; development immunosensors; single domain ... See more keywords
Physicochemical improvement of rabbit derived single-domain antibodies by substitutions with amino acids conserved in camelid antibodies.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2018.01.006
Abstract: Recently, we showed that immunized rabbit heavy chain variable regions (rVHs) can have strong antigen binding activity comparable to that of the camelid variable domain of the heavy chain of heavy chain antibody (VHH). These… read more here.
Keywords: chain; single domain; domain antibodies; rabbit ... See more keywords
Improved production of single domain antibodies with two disulfide bonds by co-expression of chaperone proteins in the Escherichia coli periplasm.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of immunological methods"
DOI: 10.1016/j.jim.2017.01.007
Abstract: Single domain antibodies are recombinantly expressed variable domains derived from camelid heavy chain antibodies. Natural single domain antibodies can have noncanonical disulfide bonds between their complementarity-determining regions that help position the binding site. In addition,… read more here.
Keywords: domain antibodies; disulfide bond; disulfide bonds; single domain ... See more keywords
Intracellular Neutralization of Ricin Toxin by Single Domain Antibodies Targeting the Active Site.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2020.01.006
Abstract: The extreme potency of the plant toxin, ricin, is due to its enzymatic subunit, RTA, which inactivates mammalian ribosomes with near perfect efficiency. Here we characterized, at the functional and structural levels, seven alpaca single-domain… read more here.
Keywords: single domain; domain antibodies; active site; site ... See more keywords
Yeast Display Enables Identification of Covalent Single-Domain Antibodies against Botulinum Neurotoxin Light Chain A.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00574
Abstract: While covalent drug discovery is reemerging as an important route to small-molecule therapeutic leads, strategies for the discovery and engineering of protein-based irreversible binding agents remain limited. Here, we describe the use of yeast display… read more here.
Keywords: yeast display; display; single domain; neurotoxin light ... See more keywords
Nanobody approval gives domain antibodies a boost
Sign Up to like & getrecommendations! Published in 2019 at "Nature Reviews Drug Discovery"
DOI: 10.1038/d41573-019-00104-w
Abstract: The FDA has approved a first nanobody, lifting hopes for companies that are exploring innovative uses for domain antibodies. The FDA has approved a first nanobody, lifting hopes for companies that are exploring innovative uses… read more here.
Keywords: antibodies boost; nanobody; nanobody approval; domain antibodies ... See more keywords
Discovery and characterization of single-domain antibodies for polymeric Ig receptor-mediated mucosal delivery of biologics
Sign Up to like & getrecommendations! Published in 2020 at "mAbs"
DOI: 10.1080/19420862.2019.1708030
Abstract: ABSTRACT Mucosal immunity is dominated by secretory IgA and IgM, although these are less favorable compared to IgG molecules for therapeutic development. Polymeric IgA and IgM are actively transported across the epithelial barrier via engagement… read more here.
Keywords: polymeric receptor; single domain; domain antibodies; characterization single ... See more keywords
CS1-specific single-domain antibodies labeled with Actinium-225 prolong survival and increase CD8+ T cells and PD-L1 expression in Multiple Myeloma
Sign Up to like & getrecommendations! Published in 2021 at "Oncoimmunology"
DOI: 10.1080/2162402x.2021.2000699
Abstract: ABSTRACT Multiple myeloma (MM) is a hematological malignancy characterized by the presence of clonal plasma cells in the bone marrow niche. Despite significant therapeutic advances, MM remains incurable for the majority of patients. Targeted radionuclide… read more here.
Keywords: expression; actinium 225; cs1; single domain ... See more keywords
Stability-Diversity Tradeoffs Impose Fundamental Constraints on Selection of Synthetic Human VH/VL Single-Domain Antibodies from In Vitro Display Libraries
Sign Up to like & getrecommendations! Published in 2017 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2017.01759
Abstract: Human autonomous VH/VL single-domain antibodies (sdAbs) are attractive therapeutic molecules, but often suffer from suboptimal stability, solubility and affinity for cognate antigens. Most commonly, human sdAbs have been isolated from in vitro display libraries constructed… read more here.
Keywords: vitro display; display libraries; selection; single domain ... See more keywords