Biophysical Investigation of the Interplay between the Conformational Species of Domain-Swapped GB1 Amyloid Mutant through Real–Time Monitoring of Amyloid Fibrillation
Sign Up to like & getrecommendations! Published in 2021 at "ACS Omega"
DOI: 10.1021/acsomega.1c04223
Abstract: Mutant polypeptide GB1HS#124F26A, which is known to aggregate into amyloid-like fibrils, has been utilized as a model in this study for gaining insights into the mechanism of domain-swapped aggregation through real-time monitoring. Size exclusion with… read more here.
Keywords: time; real time; aggregation; domain swapped ... See more keywords
Alkaline State of the Domain-Swapped Dimer of Human Cytochrome c: A Conformational Switch for Apoptotic Peroxidase Activity.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c08325
Abstract: A 2.08 Å structure of an alkaline conformer of the domain-swapped dimer of K72A human cytochrome c (Cytc) crystallized at pH 9.9 is presented. In the structure, Lys79 is ligated to the heme. All other… read more here.
Keywords: peroxidase activity; swapped dimer; domain swapped;
Engineering the hCRBPII domain-swapped dimer into a new class of protein switches.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.9b04664
Abstract: Protein conformational switches or allosteric proteins play a key role in the regulation of many essential biological pathways. Nonetheless, the implementation of protein conformational switches in protein design applications has proven challenging, with only a… read more here.
Keywords: domain swapped; protein conformational; metal; hcrbpii ... See more keywords
Structural characterisation of a novel GPVI nanobody-complex reveals a biologically active domain-swapped GPVI dimer.
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DOI: 10.1182/blood.2020009440
Abstract: GPVI is the major signalling receptor for collagen on platelets. We have raised 54 nanobodies (Nb), grouped into 33 structural classes based on their complementary determining region 3 (CDR3) loops, against recombinant GPVI-Fc (dimeric GPVI)… read more here.
Keywords: collagen; domain swapped; domain swap; recombinant gpvi ... See more keywords
The regulatory domains of the lipid exporter ABCA1 form domain swapped latches
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DOI: 10.1371/journal.pone.0262746
Abstract: ABCA1 and ABCA4 are enigmatic because they transport substrates in opposite directions yet share >50% amino acid identity. ABCA4 imports lipid conjugates but ABCA1 exports lipids. Both hydrolyze ATP to drive transport, and both contain… read more here.
Keywords: domain swapped; exporter; domain; regulatory domains ... See more keywords