Expression and Purification of SH2 Domains Using Baculovirus Expression System.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6762-9_11
Abstract: Recombinant proteins expressed in bacteria are sometimes insoluble, aggregated, and incorrectly folded. For those Src homology 2 (SH2) domains that are insoluble in bacteria, baculovirus-insect cell expression systems can be an alternative to produce soluble… read more here.
Keywords: sh2 domains; domains using; baculovirus expression; expression system ... See more keywords
High-Throughput Isolation of Soluble Protein Domains Using a Bipartite Split-GFP Complementation System.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9624-7_15
Abstract: The identification of soluble, folded domains of proteins is a recurring task in modern molecular biology. We detail a protocol for identifying compact soluble protein domains using a self-assembling two-part split-GFP comprised of a detector… read more here.
Keywords: protein; split gfp; soluble protein; domains using ... See more keywords
Detecting Phishing Domains Using Machine Learning
Sign Up to like & getrecommendations! Published in 2023 at "Applied Sciences"
DOI: 10.3390/app13084649
Abstract: Phishing is an online threat where an attacker impersonates an authentic and trustworthy organization to obtain sensitive information from a victim. One example of such is trolling, which has long been considered a problem. However,… read more here.
Keywords: using machine; machine learning; phishing domains; domains using ... See more keywords