pH‐Responsive Polymer Nanoparticles for Efficient Delivery of Cas9 Ribonucleoprotein With or Without Donor DNA
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Materials"
DOI: 10.1002/adma.202110618
Abstract: Clustered regularly interspaced short palindromic repeat (CRISPR)‐associated protein 9 (Cas9) may offer new therapeutics for genetic diseases through gene disruption via nonhomologous end joining (NHEJ) or gene correction via homology‐directed repair (HDR). However, clinical translation… read more here.
Keywords: responsive polymer; efficient delivery; donor dna; cas9 ribonucleoprotein ... See more keywords
Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair
Sign Up to like & getrecommendations! Published in 2017 at "Nature biomedical engineering"
DOI: 10.1038/s41551-017-0137-2
Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR associated protein 9 (Cas9)-based therapeutics, especially those that can correct gene mutations via homology-directed repair, have the potential to revolutionize the treatment of genetic diseases. However, it is… read more here.
Keywords: donor dna; cas9 ribonucleoprotein; homology directed; dna ... See more keywords
Improving Precise Genome Editing Using Donor DNA/gRNA Hybrid Duplex Generated by Complementary Bases
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DOI: 10.3390/biom12111621
Abstract: In precise genome editing, site-specific DNA double-strand breaks (DSBs) induced by the CRISPR/Cas9 system are repaired via homology-directed repair (HDR) using exogenous donor DNA templates. However, the low efficiency of HDR-mediated genome editing is a… read more here.
Keywords: genome editing; dna; donor dna; efficiency ... See more keywords