Dot Blot Analysis for Measuring Global N6-Methyladenosine Modification of RNA.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8808-2_20
Abstract: Posttranscriptional modification of mRNAs plays an important role in establishing the functional diversity of the proteome. The m6A modification is found in many species of RNA, including tRNA, mRNA, rRNA, and long noncoding RNAs. The physiological role… read more here.
Keywords: dot blot; modification; m6a modification; m6a ... See more keywords
Conformational Change in Herpes Simplex Virus Entry Glycoproteins Detected by Dot Blot.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9814-2_18
Abstract: Conformational changes in viral membrane proteins drive membrane fusion, a critical step in virus entry and infection. Here we describe a simple and rapid virus blotting immunoassay to define conformational changes with a panel of… read more here.
Keywords: dot blot; virus entry; herpes simplex; simplex virus ... See more keywords
Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection
Sign Up to like & getrecommendations! Published in 2021 at "Tropical Plant Pathology"
DOI: 10.1007/s40858-020-00379-0
Abstract: Tomato chlorosis virus (ToCV) is a whitefly-transmitted crinivirus that causes yield losses, mainly in tomato (Solanum lycopersicum) and potato (S. tuberosum) crops. In this work, a polyclonal antiserum for detecting the virus using a dot-blot… read more here.
Keywords: potato; virus; blot immunoassay; potato genotypes ... See more keywords
Quantitative Dot Blot (QDB) as a universal platform for absolute quantification of tissue biomarkers.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2019.04.003
Abstract: To circumvent the limitations associated with sandwich ELISA for tissue biomarker quantitation, Quantitative Dot Blot method (QDB) was proposed using antibodies clinically validated for immunohistochemistry (IHC), as this method requires only one primary antibody in… read more here.
Keywords: quantitative dot; dot blot; tissue biomarkers; tissue ... See more keywords
Effect of storage and processing on the immunodetectability of fish proteins using pooled monoclonal antibodies in ELISA and dot blot
Sign Up to like & getrecommendations! Published in 2021 at "Food Control"
DOI: 10.1016/j.foodcont.2021.107976
Abstract: Abstract Fish are listed as one of the “big-eight” major allergenic food groups. A previously developed indirect enzyme-linked immunosorbent assay (iELISA) based on three pooled monoclonal antibodies (mAbs, including 2G9, 2F3 and 8F5) reliably can… read more here.
Keywords: fish proteins; monoclonal antibodies; storage; pooled monoclonal ... See more keywords
[Validation of the technique based on Dot blot for the detection of Cercospora kikuchii in soybean plants].
Sign Up to like & getrecommendations! Published in 2018 at "Revista Argentina de microbiologia"
DOI: 10.1016/j.ram.2017.06.003
Abstract: Cercospora kikuchii is a common pathogen in soybean plants that causes crop spoilage. Its early and precise identification would prevent the misuse of pesticides and allow the initiation of an appropriate treatment. A quick, economical… read more here.
Keywords: technique; dot blot; soybean plants; cercospora kikuchii ... See more keywords
Genotyping of Malaysian G6PD-deficient neonates by reverse dot blot flow-through hybridisation
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Human Genetics"
DOI: 10.1038/s10038-019-0700-7
Abstract: G6PD deficiency is the commonest enzyme deficiency found in humans. Current diagnostic methods lack sensitivity to detect all cases of G6PD deficiency. We evaluated the reverse dot blot flow-through hybridisation assay designed to detect simultaneously… read more here.
Keywords: blot flow; g6pd; reverse dot; dot blot ... See more keywords
Performance of the Dot-blot test method for detecting antibodies to Sarcocystis spp. in cattle
Sign Up to like & getrecommendations! Published in 2020 at "Pesquisa Veterinaria Brasileira"
DOI: 10.1590/1678-5150-pvb-6521
Abstract: ABSTRACT: Serological techniques can detect antibodies against Sarcocystis spp., Neospora caninum and Toxoplasma gondii antigens in single or mixed infections. Immunofluorescent antibody tests (IFAT) is considered the gold standard technique for Sarcocystosis diagnostic in cattle… read more here.
Keywords: dot blot; caninum gondii; antibodies sarcocystis; sarcocystis spp ... See more keywords
Development and evaluation of polyclonal antibody-based antigen detection ELISA and dot blot assays as a less costly diagnostic alternative for pathogenic leptospira infection.
Sign Up to like & getrecommendations! Published in 2022 at "Acta tropica"
DOI: 10.2139/ssrn.4223122
Abstract: Leptospirosis is an infectious, zoonotic disease of worldwide distribution, the cause of which is infection by pathogenic leptospira. In Chile, dairy cattle are recognized a significant source in the maintenance and transmission of this infection,… read more here.
Keywords: pathogenic leptospira; infection; dot blot;