Dissipation of double-stranded RNA in aquatic microcosms.
Sign Up to like & getrecommendations! Published in 2017 at "Environmental toxicology and chemistry"
DOI: 10.1002/etc.3648
Abstract: Silencing genes of a pest with double-stranded RNA (dsRNA) is a promising new pest management technology. As part of the environmental risk assessment for dsRNA-based products, the environmental fate and the potential for adverse effects… read more here.
Keywords: dissipation double; water; double stranded; stranded rna ... See more keywords
Reproducible Enhancement of Fluorescence by Bimetal Mediated Surface Plasmon Coupled Emission for Highly Sensitive Quantitative Diagnosis of Double-Stranded DNA.
Sign Up to like & getrecommendations! Published in 2018 at "Small"
DOI: 10.1002/smll.201801385
Abstract: Plasmonic enhancement of fluorescence from SYBR Green I conjugated with a double-stranded DNA (dsDNA) amplicon is demonstrated on polymerase chain reaction (PCR) products. Theoretical computation leads to use of the bimetallic (Au 2 nm-Ag 50… read more here.
Keywords: enhancement fluorescence; dna; fluorescence; surface ... See more keywords
Universal Library Preparation Protocol for Efficient High-Throughput Sequencing of Double-Stranded RNA Viruses.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0138-9_14
Abstract: This chapter reports a library preparation protocol for efficient high-throughput sequencing of double-stranded RNA viruses. The protocol consists of four main steps, viz., enzyme treatment, precipitation using lithium chloride, full-length amplification of cDNAs, and tailing… read more here.
Keywords: rna viruses; double stranded; stranded rna; throughput sequencing ... See more keywords
Comet Assay for the Detection of Single and Double-Strand DNA Breaks.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1948-3_18
Abstract: DNA damage is a common event that occurs during the life span of the majority of cells, as a result of aberrant energy metabolism, exposure to radiation, DNA damaging chemicals, and viral infections. Rapid, sensitive,… read more here.
Keywords: dna; single double; double stranded; comet assay ... See more keywords
Viral Double-Stranded RNAs (dsRNAs) from Plants: Alternative Nucleic Acid Substrates for High-Throughput Sequencing.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7683-6_4
Abstract: High-throughput sequencing (or next-generation sequencing-NGS) is an emerging technology that allows the detection of plant viruses without any prior knowledge. Various sequencing techniques and various templates can be used as substrate for NGS. This chapter… read more here.
Keywords: double stranded; stranded rnas; rnas dsrnas; throughput sequencing ... See more keywords
Establishment and application of a loop-mediated isothermal amplification method with double-stranded displacement probes to quantify the genetically modified rice M12 event
Sign Up to like & getrecommendations! Published in 2020 at "European Food Research and Technology"
DOI: 10.1007/s00217-020-03430-1
Abstract: For the purpose of supervision and academic research on GMO, it was necessary to establish a simple and effective on-site qualitative and quantitative method for GMO detection. In this paper, double-stranded displacement probes were first… read more here.
Keywords: m12 event; displacement probes; rice m12; double stranded ... See more keywords
Double-Stranded RNA-Mediated Suppression of Trypsin-Like Serine Protease (t-SP) Triggers Over-Expression of Another t-SP Isoform in Helicoverpa armigera
Sign Up to like & getrecommendations! Published in 2017 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-017-2584-3
Abstract: High diversity of digestive proteases is considered to be the key factor in the evolution of polyphagy in Helicoverpa armigera. Serine proteases (SPs) contribute ~85% of the dietary protein digestion in H. armigera. We investigated… read more here.
Keywords: helicoverpa armigera; isoform; double stranded; stranded rna ... See more keywords
Specific and robust hybridization based on double-stranded nucleic acids with single-base resolution.
Sign Up to like & getrecommendations! Published in 2022 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2022.339568
Abstract: Nucleic acid hybridization plays a critical role in medical diagnostics and nanotechnology, but its selectivity and robustness remain to be improved. Here, focusing on double-stranded nucleic acid-based hybridization, we present a series of related strategies.… read more here.
Keywords: hybridization; double stranded; nucleic acids; stranded nucleic ... See more keywords
Double-stranded RNA in exosomes: Potential systemic RNA interference pathway in the Colorado potato beetle, Leptinotarsa decemlineata
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Asia-pacific Entomology"
DOI: 10.1016/j.aspen.2020.09.012
Abstract: Abstract Despite extensive research during the past decade elucidating the mechanism of RNA interference (RNAi) in insects, it is not clear how ingested or injected double-stranded RNA (dsRNA) triggers RNAi response in the whole body… read more here.
Keywords: double stranded; rna interference; lepd sl1; rnai ... See more keywords
Double-stranded RNA-dependent kinase PKR activates NF-κB pathway in acute pancreatitis.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2018.07.080
Abstract: The activation of transcription factor nuclear factor kappa B (NF-κB) occurs early in acute pancreatitis (AP) simultaneously with intracellular trypsinogen activation. Double-stranded RNA-dependent kinase (PKR) promotes the activation of NF-κB and the production of pro-inflammatory… read more here.
Keywords: acute pancreatitis; kinase; ar42j cells; double stranded ... See more keywords
An electrochemical study of 9-chloroacridine redox behavior and its interaction with double-stranded DNA.
Sign Up to like & getrecommendations! Published in 2020 at "Bioelectrochemistry"
DOI: 10.1016/j.bioelechem.2020.107579
Abstract: The electrochemical behavior of 9-chloroacridine (9Cl-A), a precursor molecule for synthesis of acridine derivatives with cytostatic activity, is a complex, pH-dependent, diffusion-controlled irreversible process. Oxidation of 9Cl-A initiates with the formation of a cation radical… read more here.
Keywords: dna; electrochemical study; double stranded; chloroacridine ... See more keywords