Evaluating digital PCR for the quantification of human nuclear DNA: determining target strandedness
Sign Up to like & getrecommendations! Published in 2020 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-020-02733-2
Abstract: Digital polymerase chain reaction (dPCR) methodology has been asserted to be a “potentially primary” analytical approach for assigning DNA concentration. The essence of dPCR measurements is the independent dispersal of fragments into multiple reaction partitions,… read more here.
Keywords: dna; evaluating digital; target; human nuclear ... See more keywords
Microwell Array-based Digital PCR for Influenza Virus Detection
Sign Up to like & getrecommendations! Published in 2019 at "BioChip Journal"
DOI: 10.1007/s13206-019-3302-8
Abstract: Digital polymerase chain reaction (dPCR) enables the accurate determination of deoxyribonucleic acid (DNA) copy numbers. In contrast to real-time PCR (qPCR), dPCR can perform quantification without requiring previous standard curve experiments. Furthermore, dPCR is more… read more here.
Keywords: pcr; influenza virus; dpcr; microwell ... See more keywords
Virtual-Partition Digital PCR for High-Precision Chromosomal Counting Applications.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c03527
Abstract: Digital PCR (dPCR) is the gold-standard analytical platform for rapid high-precision quantification of genomic fragments. However, current dPCR assays are generally limited to monitoring 1-2 analytes per sample, thereby limiting the platform's ability to address… read more here.
Keywords: partition; virtual partition; high precision; dpcr ... See more keywords
Digital PCR and its applications in noninvasive prenatal testing.
Sign Up to like & getrecommendations! Published in 2022 at "Briefings in functional genomics"
DOI: 10.1093/bfgp/elac024
Abstract: In the past decade, digital PCR (dPCR), as a new nucleic acid absolute quantification technology, has been widely used in clinical research. dPCR does not rely on the standard curve and has a higher tolerance… read more here.
Keywords: noninvasive prenatal; prenatal testing; dpcr; pcr applications ... See more keywords
Use of Pleural Fluid Digital PCR Analysis to Improve the Diagnosis of Pleural Tuberculosis
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DOI: 10.1128/spectrum.01632-22
Abstract: Pleural TB is one of the most frequent causes of pleural effusion, especially in areas with high burden of TB. Due to the paucibacillary nature of the disease, the diagnostic sensitivities of all available bacteriological… read more here.
Keywords: dpcr; is1081 dpcr; diagnosis; digital pcr ... See more keywords