A poly(dimethylsiloxane) microfluidic sheet reversibly adhered on a glass plate for creation of emulsion droplets for droplet digital PCR
Sign Up to like & getrecommendations! Published in 2017 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201600309
Abstract: A PDMS microfluidic chip with T‐junction channel geometry, two inlet reservoirs, and one outlet reservoir was reversibly adhered on a glass plate through the viscoelastic properties of PDMS. This formed a detachable microfluidic device for… read more here.
Keywords: glass plate; creation; droplet digital; glass ... See more keywords
Quantitative detections of TP53 gene mutations improve the diagnosis and prognostic prediction of biliary tract cancers using droplet digital PCR.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of clinical laboratory analysis"
DOI: 10.1002/jcla.24103
Abstract: OBJECTIVE Biliary tract cancer (BTC) is a rare malignancy and lack of effective diagnostic and prognostic marker. Here, we aimed to investigate the clinical implication of TP53 mutation detection in BTC using droplet digital PCR (ddPCR).… read more here.
Keywords: biliary tract; droplet digital; btc; digital pcr ... See more keywords
Droplet‐digital PCR assay to detect Merkel cell polyomavirus sequences in chorionic villi from spontaneous abortion affected females
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Cellular Physiology"
DOI: 10.1002/jcp.29213
Abstract: Droplet‐digital polymerase chain reaction (ddPCR) technique was set up to detect/quantify Merkel cell polyomavirus (MCPyV) DNA in clinical specimens, including chorionic villi and peripheral blood mononuclear cells (PBMCs) from spontaneous abortion (SA)‐affected females. This ddPCR… read more here.
Keywords: chorionic villi; droplet digital; dna; merkel cell ... See more keywords
An effective analytical droplet digital PCR approach for identification and quantification of fur-bearing animal meat in raw and processed food.
Sign Up to like & getrecommendations! Published in 2021 at "Food chemistry"
DOI: 10.1016/j.foodchem.2021.129525
Abstract: Available nuclear gene sequences for meat detection are still rare and little applicability in the investigation of new types of meat adulteration such as fox, mink and raccoon dog was performed. In the present work,… read more here.
Keywords: droplet digital; animal meat; bearing animal; meat ... See more keywords
Absolute quantification of Vibrio parahaemolyticus by multiplex droplet digital PCR for simultaneous detection of tlh, tdh and ureR based on single intact cell
Sign Up to like & getrecommendations! Published in 2020 at "Food Control"
DOI: 10.1016/j.foodcont.2020.107207
Abstract: Abstract Vibrio parahaemolyticus, a marine food-borne pathogen, has been proved to be a significant cause of human gastrointestinal disorders worldwide. In this study, a method of multiplex droplet digital PCR (ddPCR) based on primer and… read more here.
Keywords: vibrio parahaemolyticus; detection; droplet digital; pcr ... See more keywords
Determination of SARS-CoV-2 Contamination in a Neonatal Intensive Care Unit (NICU) Environment Using Droplet Digital PCR (ddPCR)
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Infectious Diseases"
DOI: 10.1016/j.ijid.2021.12.125
Abstract: Purpose Neonatal infections with SARS-CoV-2 are thought to be less contagious than in older children and adults. The transmission of SARS-CoV-2 from neonates and their environment has not been well studied. Droplet Digital PCR (ddPCR)… read more here.
Keywords: incubator; droplet; sars cov; environment ... See more keywords
Optimisation of droplet digital PCR for determining copy number variation of α-gliadin genes in mutant and gene-edited polyploid bread wheat
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Cereal Science"
DOI: 10.1016/j.jcs.2019.102903
Abstract: Targeted and random mutagenesis of gene families require accurate quantification. Droplet digital PCR (ddPCR) enables high-throughput screening of copy number variation (CNV). We tested the accuracy of ddPCR for CNV analysis in the large α-gliadin… read more here.
Keywords: ddpcr; gliadin genes; droplet digital; wheat ... See more keywords
Novel multiplex droplet digital PCR assay for scoring PD-L1 in non-small cell lung cancer biopsy specimens.
Sign Up to like & getrecommendations! Published in 2019 at "Lung cancer"
DOI: 10.1016/j.lungcan.2019.06.029
Abstract: OBJECTIVES Immune checkpoint inhibitors have become integrated into the clinical management of non-small cell lung cancer (NSCLC). Using RTqPCR, we have previously identified a gene expression panel that detected presence of malignant cells (MMP9:TIMP3 ratio)… read more here.
Keywords: biopsy specimens; droplet digital; lung cancer; cell ... See more keywords
Development and comparative evaluation of droplet digital PCR and quantitative PCR for the detection and quantification of Chlamydia psittaci.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2021.106318
Abstract: Chlamydia psittaci is a zoonotic pathogen mainly transmitted by psittacine birds and poultry. The low shedding rate of the pathogen in the apparently healthy birds and human clinical cases may result in false-negative results. In… read more here.
Keywords: detection; droplet digital; ddpcr assay; pcr ... See more keywords
Measuring KRAS Mutations in Circulating Tumor DNA by Droplet Digital PCR and Next-Generation Sequencing
Sign Up to like & getrecommendations! Published in 2018 at "Translational Oncology"
DOI: 10.1016/j.tranon.2018.07.013
Abstract: Measuring total cell-free DNA (cfDNA) or cancer-specific mutations herein has presented as new tools in aiding the treatment of cancer patients. Studies show that total cfDNA bears prognostic value in metastatic colorectal cancer (mCRC) and… read more here.
Keywords: dna; droplet digital; cancer; next generation ... See more keywords
Droplet digital PCR applications in the tuberculosis world.
Sign Up to like & getrecommendations! Published in 2019 at "Tuberculosis"
DOI: 10.1016/j.tube.2019.07.001
Abstract: Droplet digital polymerase chain reaction (ddPCR) is a third generation of polymerase chain reaction (PCR) that enables the exact quantification of nucleic acid targets within a sample. The capability of ddPCR to accurately detect and… read more here.
Keywords: applications tuberculosis; droplet digital; pcr; tuberculosis ... See more keywords