Fluorescence-Activated Droplet Sorting for Single-Cell Directed Evolution.
Sign Up to like & getrecommendations! Published in 2019 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.9b00103
Abstract: Synthetic biology aims to improve human health and the environment by repurposing biological enzymes for use in practical applications. However, natural enzymes often function with suboptimal activity when engineered into biological pathways or challenged to… read more here.
Keywords: droplet sorting; biology; activated droplet; directed evolution ... See more keywords
High-throughput screening of cell-free riboswitches by fluorescence-activated droplet sorting
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac152
Abstract: Abstract Cell-free systems that display complex functions without using living cells are emerging as new platforms to test our understanding of biological systems as well as for practical applications such as biosensors and biomanufacturing. Those… read more here.
Keywords: cell; cell free; droplet sorting; throughput screening ... See more keywords
Method for Passive Droplet Sorting after Photo-Tagging
Sign Up to like & getrecommendations! Published in 2020 at "Micromachines"
DOI: 10.3390/mi11110964
Abstract: We present a method to photo-tag individual microfluidic droplets for latter selection by passive sorting. The use of a specific surfactant leads to the interfacial tension to be very sensitive to droplet pH. The photoexcitation… read more here.
Keywords: droplet; photo; selection; method passive ... See more keywords