Pooled genome-wide CRISPR activation screening for rapamycin resistance genes in Drosophila cells
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.1101/2022.12.09.519790
Abstract: Loss-of-function and gain-of-function genetic perturbations provide valuable insights into gene function. In Drosophila cells, while genome-wide loss-of-function screens have been extensively used to reveal mechanisms of a variety of biological processes, approaches for performing genome-wide… read more here.
Keywords: rapamycin resistance; function; genome wide; drosophila cells ... See more keywords
RNAi in Drosophila S2 Cells by dsRNA Soaking.
Sign Up to like & getrecommendations! Published in 2019 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot097477
Abstract: This is a simple method for inducing RNAi in Drosophila S2 cells by soaking the cells in medium containing dsRNAs. In comparison with transfection, soaking requires fewer procedures and avoids the cost of transfection reagents,… read more here.
Keywords: rnai drosophila; transfection; dsrna soaking; cells dsrna ... See more keywords
RNAi in Drosophila S2 Cells by siRNA Duplex or dsRNA Transfection.
Sign Up to like & getrecommendations! Published in 2019 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot097485
Abstract: This protocol describes an efficient method to trigger RNAi in Drosophila S2 cells by using transfection reagents to deliver siRNAs or dsRNAs. read more here.
Keywords: cells sirna; rnai drosophila; transfection; duplex dsrna ... See more keywords
Translational Control of Serrate Expression in Drosophila Cells
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DOI: 10.21873/invivo.12326
Abstract: Background/Aim: The DSL proteins, Serrate and Delta, which act as Notch receptor ligands, mediate signalling between adjacent cells, when a ligand-expressing cell binds to Notch on an adjacent receiving cell. Notch is ubiquitously expressed and… read more here.
Keywords: serrate expression; control serrate; translational control; expression drosophila ... See more keywords