Reconstructing ERK Signaling in the Drosophila Embryo from Fixed Images.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6424-6_25
Abstract: The early Drosophila embryo provides unique opportunities for quantitative studies of ERK signaling. This system is characterized by simple anatomy, the ease of obtaining large numbers of staged embryos, and the availability of powerful tools… read more here.
Keywords: drosophila embryo; erk activation; reconstructing erk; embryo ... See more keywords
Studying Nonproliferative Roles for Egfr Signaling in Tissue Morphogenesis Using Dorsal Closure of the Drosophila Embryo.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7219-7_16
Abstract: For several decades, genetic analysis in Drosophila has made important contributions to the understanding of signaling by Egfr. Egfr has been well characterized with regard to its oncogenic potential but is also being studied for… read more here.
Keywords: drosophila embryo; closure; egfr signaling; dorsal closure ... See more keywords
Excess histone H3 is a competitive Chk1 inhibitor that controls cell-cycle remodeling in the early Drosophila embryo
Sign Up to like & getrecommendations! Published in 2021 at "Current Biology"
DOI: 10.1016/j.cub.2021.03.035
Abstract: The DNA damage checkpoint is crucial to protect genome integrity.1,2 However, the early embryos of many metazoans sacrifice this safeguard to allow for rapid cleavage divisions that are required for speedy development. At the mid-blastula… read more here.
Keywords: chk1; chk1 inhibitor; drosophila embryo; cell cycle ... See more keywords
Combined modelling of mRNA decay dynamics and single-molecule imaging in the Drosophila embryo uncovers a role for P-bodies in 5′ to 3′ degradation
Sign Up to like & getrecommendations! Published in 2023 at "PLOS Biology"
DOI: 10.1371/journal.pbio.3001956
Abstract: Regulation of mRNA degradation is critical for a diverse array of cellular processes and developmental cell fate decisions. Many methods for determining mRNA half-lives rely on transcriptional inhibition or metabolic labelling. Here, we use a… read more here.
Keywords: single molecule; molecule imaging; drosophila embryo; mrna ... See more keywords
Mechanical feedback and robustness of apical constrictions in Drosophila embryo ventral furrow formation
Sign Up to like & getrecommendations! Published in 2021 at "PLoS Computational Biology"
DOI: 10.1371/journal.pcbi.1009173
Abstract: Formation of the ventral furrow in the Drosophila embryo relies on the apical constriction of cells in the ventral region to produce bending forces that drive tissue invagination. In our recent paper we observed that… read more here.
Keywords: drosophila embryo; ventral furrow; furrow formation; formation ... See more keywords
The histone chaperone NASP maintains H3-H4 reservoirs in the early Drosophila embryo
Sign Up to like & getrecommendations! Published in 2022 at "PLOS Genetics"
DOI: 10.1371/journal.pgen.1010682
Abstract: Histones are essential for chromatin packaging and histone supply must be tightly regulated as excess histones are toxic. To drive the rapid cell cycles of the early embryo, however, excess histones are maternally deposited. Therefore,… read more here.
Keywords: histone chaperone; drosophila embryo; nasp; histone ... See more keywords