Lighting Up Gene Activation in Living Drosophila Embryos.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9674-2_5
Abstract: With its rapid development, ease of collection, and the presence of a unique layer of nuclei located close to the surface, the Drosophila syncytial embryo is ideally suited to study the establishment of gene expression… read more here.
Keywords: drosophila embryos; living drosophila; lighting gene; gene ... See more keywords
Self-Organized Nuclear Positioning Synchronizes the Cell Cycle in Drosophila Embryos
Sign Up to like & getrecommendations! Published in 2019 at "Cell"
DOI: 10.1016/j.cell.2019.03.007
Abstract: The synchronous cleavage divisions of early embryogenesis require coordination of the cell-cycle oscillator, the dynamics of the cytoskeleton, and the cytoplasm. Yet, it remains unclear how spatially restricted biochemical signals are integrated with physical properties of… read more here.
Keywords: drosophila embryos; cell cycle; cycle; cycle drosophila ... See more keywords
mRNA quantification using single-molecule FISH in Drosophila embryos
Sign Up to like & getrecommendations! Published in 2017 at "Nature Protocols"
DOI: 10.1038/nprot.2017.030
Abstract: Spatial information is critical to the interrogation of developmental and tissue-level regulation of gene expression. However, this information is usually lost when global mRNA levels from tissues are measured using reverse transcriptase PCR, microarray analysis… read more here.
Keywords: drosophila; drosophila embryos; single molecule; mrna ... See more keywords
Bivalency in Drosophila embryos is associated with strong inducibility of Polycomb target genes
Sign Up to like & getrecommendations! Published in 2019 at "Fly"
DOI: 10.1080/19336934.2019.1619438
Abstract: ABSTRACT Polycomb group (PcG) and Trithorax group (TrxG) proteins orchestrate development of a multicellular organism by faithfully maintaining cell fate decisions made early in embryogenesis. An important chromatin mark connected to PcG/TrxG regulation is bivalent… read more here.
Keywords: bivalency; polycomb; drosophila embryos; inducibility ... See more keywords
Quantitative microscopy uncovers ploidy changes during mitosis in live Drosophila embryos and their effect on nuclear size
Sign Up to like & getrecommendations! Published in 2017 at "Biology Open"
DOI: 10.1242/bio.022079
Abstract: ABSTRACT Time-lapse microscopy is a powerful tool to investigate cellular and developmental dynamics. In Drosophila melanogaster, it can be used to study division cycles in embryogenesis. To obtain quantitative information from 3D time-lapse data and… read more here.
Keywords: microscopy; drosophila embryos; size; quantitative microscopy ... See more keywords
Dynamics of cortical domains in early Drosophila development
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Cell Science"
DOI: 10.1242/jcs.212795
Abstract: ABSTRACT Underlying the plasma membrane of eukaryotic cells is an actin cortex that includes actin filaments and associated proteins. A special feature of all polarized and epithelial cells are cortical domains, each of which is… read more here.
Keywords: cortical domains; drosophila embryos; dynamics cortical; early drosophila ... See more keywords
Interphase-arrested Drosophila embryos activate zygotic gene expression and initiate mid-blastula transition events at a low nuclear-cytoplasmic ratio
Sign Up to like & getrecommendations! Published in 2020 at "PLoS Biology"
DOI: 10.1371/journal.pbio.3000891
Abstract: Externally deposited eggs begin development with an immense cytoplasm and a single overwhelmed nucleus. Rapid mitotic cycles restore normality as the ratio of nuclei to cytoplasm (N/C) increases. A threshold N/C has been widely proposed… read more here.
Keywords: cyclin cdk1; drosophila embryos; ratio; blastula transition ... See more keywords