Phenotypic Analysis of ATM Protein Kinase in DNA Double-Strand Break Formation and Repair.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6955-5_23
Abstract: Ataxia telangiectasia mutated (ATM) encodes a serine/threonine protein kinase, which is involved in various regulatory processes in mammalian cells. Its best-known role is apical activation of the DNA damage response following generation of DNA double-strand… read more here.
Keywords: dna; protein kinase; repair; atm ... See more keywords
Quantitative assessment of HR and NHEJ activities via CRISPR/Cas9-induced oligodeoxynucleotide-mediated DSB repair.
Sign Up to like & getrecommendations! Published in 2018 at "DNA repair"
DOI: 10.1016/j.dnarep.2018.09.002
Abstract: Homologous recombination (HR) and non-homologous end joining (NHEJ) are the two major mechanisms for the repair of DNA double-strand breaks (DSBs) in eukaryotic cells. Previously, we designed an assay for detecting NHEJ activity by using… read more here.
Keywords: nhej activities; repair; crispr cas9; cas9 ... See more keywords
Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response
Sign Up to like & getrecommendations! Published in 2022 at "Cell Research"
DOI: 10.1038/s41422-021-00597-4
Abstract: In response to DNA double-strand breaks (DSBs), DNA damage repair factors are recruited to DNA lesions and form nuclear foci. However, the underlying molecular mechanism remains largely elusive. Here, by analyzing the localization of DSB… read more here.
Keywords: pre; repair; repair factors; dsb repair ... See more keywords
HELQ is a dual-function DSB repair enzyme modulated by RPA and RAD51
Sign Up to like & getrecommendations! Published in 2021 at "Nature"
DOI: 10.1038/s41586-021-04261-0
Abstract: DNA double-stranded breaks (DSBs) are deleterious lesions, and their incorrect repair can drive cancer development 1 . HELQ is a superfamily 2 helicase with 3′ to 5′ polarity, and its disruption in mice confers germ… read more here.
Keywords: dna; rad51; strand annealing; rpa rad51 ... See more keywords
Efficient DNA double-strand break formation at single or multiple defined sites in the Saccharomyces cerevisiae genome
Sign Up to like & getrecommendations! Published in 2020 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa833
Abstract: Abstract DNA double-strand breaks (DSBs) are common genome lesions that threaten genome stability and cell survival. Cells use sophisticated repair machineries to detect and heal DSBs. To study DSB repair pathways and associated factors, inducible… read more here.
Keywords: saccharomyces cerevisiae; dna double; formation; double strand ... See more keywords
Dipeptide repeat proteins inhibit homology-directed DNA double strand break repair in C9ORF72 ALS/FTD
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Neurodegeneration"
DOI: 10.1186/s13024-020-00365-9
Abstract: Background The C9ORF72 hexanucleotide repeat expansion is the most common known genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), two fatal age-related neurodegenerative diseases. The C9ORF72 expansion encodes five dipeptide repeat proteins… read more here.
Keywords: repair pathways; dna; homology directed; dna dsb ... See more keywords
Variants of the human RAD52 gene confer defects in ionizing radiation resistance and homologous recombination repair in budding yeast
Sign Up to like & getrecommendations! Published in 2020 at "Microbial Cell"
DOI: 10.15698/mic2020.10.732
Abstract: RAD52 is a structurally and functionally conserved component of the DNA double-strand break (DSB) repair apparatus from budding yeast to humans. We recently showed that expressing the human gene, HsRAD52 in rad52 mutant budding yeast… read more here.
Keywords: budding yeast; ionizing radiation; hrr; repair ... See more keywords
Heterogeneity of Organization of Subcompartments in DSB Repair Foci
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Genetics"
DOI: 10.3389/fgene.2022.887088
Abstract: Cells assemble compartments around DNA double-strand breaks (DSBs). The assembly of this compartment is dependent on the phosphorylation of histone H2AX, the binding of MDC1 to phosphorylated H2AX, and the assembly of downstream signaling and… read more here.
Keywords: brca1; heterogeneity; dna; array ... See more keywords
Detection of DNA Double Strand Breaks by γH2AX Does Not Result in 53bp1 Recruitment in Mouse Retinal Tissues
Sign Up to like & getrecommendations! Published in 2018 at "Frontiers in Neuroscience"
DOI: 10.3389/fnins.2018.00286
Abstract: Gene editing is an attractive potential treatment of inherited retinopathies. However, it often relies on endogenous DNA repair. Retinal DNA repair is incompletely characterized in humans and animal models. We investigated recruitment of the double… read more here.
Keywords: detection dna; dna; dna double; repair ... See more keywords
A Comprehensive Analysis of Alterations in DNA Damage Repair Pathways Reveals a Potential Way to Enhance the Radio-Sensitivity of Esophageal Squamous Cell Cancer
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Oncology"
DOI: 10.3389/fonc.2020.575711
Abstract: Esophageal squamous cell cancer (ESCC) is a common malignancy with a poor 5-year overall survival in China. Altered DNA damage repair (DDR) pathways are associated with a predisposition to cancer and contribute to therapeutic response… read more here.
Keywords: repair pathways; cancer; gene; cell ... See more keywords
Recent Advances in the Development of Non-PIKKs Targeting Small Molecule Inhibitors of DNA Double-Strand Break Repair
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Oncology"
DOI: 10.3389/fonc.2022.850883
Abstract: The vast majority of cancer patients receive DNA-damaging drugs or ionizing radiation (IR) during their course of treatment, yet the efficacy of these therapies is tempered by DNA repair and DNA damage response (DDR) pathways.… read more here.
Keywords: dna; non pikks; double strand; cancer ... See more keywords