Accelerated delivery of dsRNA in lepidopteran midgut cells by a Galanthus nivalis lectin (GNA)-dsRNA-binding domain fusion protein.
Sign Up to like & getrecommendations! Published in 2021 at "Pesticide biochemistry and physiology"
DOI: 10.1016/j.pestbp.2021.104853
Abstract: Lepidopteran insects are highly refractory to oral RNA interference (RNAi). Degradation, impaired cellular uptake and intracellular transport of double-stranded RNA (dsRNA) are considered the major factors responsible for the reduced RNAi efficiency in these insects.… read more here.
Keywords: rnai; dsrna; dsrna binding; fusion protein ... See more keywords
fCLIP-seq for transcriptomic footprinting of dsRNA-binding proteins: Lessons from DROSHA.
Sign Up to like & getrecommendations! Published in 2019 at "Methods"
DOI: 10.1016/j.ymeth.2018.06.004
Abstract: CLIP-seq (crosslinking immunoprecipitation and sequencing) is widely used to map the binding sites of a protein of interest on the transcriptome, and generally employs UV to induce the covalent bonds between protein and RNA, which… read more here.
Keywords: seq; binding proteins; drosha; dsrna binding ... See more keywords
An RNA editing/dsRNA binding-independent gene regulatory mechanism of ADARs and its clinical implication in cancer
Sign Up to like & getrecommendations! Published in 2017 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkx667
Abstract: Abstract Adenosine-to-inosine (A-to-I) RNA editing, catalyzed by Adenosine DeAminases acting on double-stranded RNA(dsRNA) (ADAR), occurs predominantly in the 3′ untranslated regions (3′UTRs) of spliced mRNA. Here we uncover an unanticipated link between ADARs (ADAR1 and… read more here.
Keywords: editing dsrna; rna; rna editing; dsrna binding ... See more keywords