Dual genetic selection of the theophylline riboswitch with altered aptamer specificity for caffeine.
Sign Up to like & getrecommendations! Published in 2021 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2021.09.058
Abstract: The aptamer domain of the theophylline riboswitch was randomized to generate a library containing millions of different variants. Dual genetic selection utilizing the cat-upp fusion gene was performed for the library, which successfully led to… read more here.
Keywords: caffeine; genetic selection; theophylline riboswitch; dual genetic ... See more keywords
Engineering Riboswitches in Vivo Using Dual Genetic Selection and Fluorescence-Activated Cell Sorting.
Sign Up to like & getrecommendations! Published in 2018 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.8b00099
Abstract: Riboswitches, noncoding RNAs that bind a small molecule effector to control gene expression at the level of transcription or translation, are uniquely suited to meet challenges in synthetic biology. To expand the limited set of… read more here.
Keywords: activated cell; genetic selection; selection fluorescence; biology ... See more keywords