Improved Production of Streptomyces sp. FA1 Xylanase in a Dual-Plasmid Pichia pastoris System.
Sign Up to like & getrecommendations! Published in 2021 at "Current issues in molecular biology"
DOI: 10.3390/cimb43030161
Abstract: Methanol is considered as a potential hazard in the methanol-induced yeast expression of food-related enzymes. To increase the production efficiency of recombinant proteins in Pichia pastoris without methanol induction, a novel dual-plasmid system was constructed, for… read more here.
Keywords: pichia pastoris; expression; system; production ... See more keywords
A Dual-Plasmid-Based CRISPR/Cas9-Mediated Strategy Enables Targeted Editing of pH Regulatory Gene pacC in a Clinical Isolate of Trichophyton rubrum
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Fungi"
DOI: 10.3390/jof8121241
Abstract: Trichophyton rubrum is the most prevalent causative agent responsible for 80–90% of all known superficial fungal infections in humans, worldwide. Limited available methods for genetic manipulations have been one of the major bottlenecks in understanding… read more here.
Keywords: crispr cas9; rubrum; plasmid; plasmid based ... See more keywords