Direct measurement of deubiquitinating enzyme activity in intact cells using a protease-resistant, cell-permeable, peptide-based reporter.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical engineering journal"
DOI: 10.1016/j.bej.2019.107320
Abstract: Deubiquitinating enzymes (DUBs) regulate the removal of the polyubiquitin chain from proteins targeted for degradation. Current approaches to quantify DUB activity are limited to test tube-based assays that incorporate enzymes or cell lysates, but not… read more here.
Keywords: cell permeable; activity; dub activity; peptide based ... See more keywords
Structure-Guided Mutagenesis Alters Deubiquitinating Activity and Attenuates Pathogenesis of a Murine Coronavirus
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DOI: 10.1128/jvi.01734-19
Abstract: Coronaviruses employ a genetic economy by encoding multifunctional proteins that function in viral replication and also modify the host environment to disarm the innate immune response. The coronavirus papain-like protease 2 (PLP2) domain possesses protease… read more here.
Keywords: activity; dub activity; virus; protease ... See more keywords
Characterizing the PRRSV nsp2 Deubiquitinase Reveals Dispensability of Cis-Activity for Replication and a Link of nsp2 to Inflammation Induction
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DOI: 10.3390/v11100896
Abstract: The papain-like cysteine protease 2 (PLP2) within the N-terminus of the porcine reproductive and respiratory syndrome virus (PRRSV) nsp2 replicase protein specifies a deubiquitinating enzyme (DUB), but its biochemical properties and the role in infection… read more here.
Keywords: dub activity; cleavage; plp2; prrsv nsp2 ... See more keywords