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   Articles with "duplex qpcr" as a keyword



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Optimization and implementation of four duplex qPCR assays for gene doping control in horseracing.

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recommendations! Published in 2022 at "Drug testing and analysis"

DOI: 10.1002/dta.3328

Abstract: The concern about gene doping has remained high in horseracing and other equestrian competitions. Our laboratory has previously developed a duplex quantitative polymerase chain reaction (qPCR) assay capable of detecting in equine blood the human… read more here.

Keywords: four duplex; gene; control; duplex qpcr ... See more keywords
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Duplex qPCR for Leishmania species identification using lesion imprint on filter paper.

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recommendations! Published in 2020 at "Experimental parasitology"

DOI: 10.1016/j.exppara.2020.108019

Abstract: BACKGROUND American cutaneous leishmaniasis (ACL) is caused by different Leishmania parasites, which stimulate and direct the immune response against the infection. OBJECTIVE To evaluate the TaqMan probe technology applicability to diagnose and identifying of Leishmania… read more here.

Keywords: lesion imprint; leishmania species; duplex qpcr; lesion ... See more keywords
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Development of a duplex qPCR system for detection and quantification of the two canola blackleg pathogens Leptosphaeria biglobosa and L. maculans.

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recommendations! Published in 2023 at "Plant disease"

DOI: 10.1094/pdis-10-22-2308-re

Abstract: Two probe-based qPCR systems, namely P-Lb and P-Lm, specific to the canola blackleg pathogens Leptosphaeria biglobosa and L. maculans, respectively, were developed and their efficiencies were tested. Each of the two systems targets a single… read more here.

Keywords: canola; two systems; biglobosa maculans; duplex qpcr ... See more keywords

Novel Duplex RT-qPCR for animal rabies surveillance.

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recommendations! Published in 2022 at "Transboundary and emerging diseases"

DOI: 10.1111/tbed.14565

Abstract: Rabies is a lethal zoonosis affecting mammals worldwide. Diagnosis of rabies follows international standard protocols, primarily relying on direct immunofluorescence (DI) followed by mouse inoculation test (MIT). WHO recommends molecular biology techniques such as RT-qPCR… read more here.

Keywords: surveillance; qpcr animal; qpcr; mit ... See more keywords