A dual-signaling electrochemical ratiometric strategy combining "signal-off" and "signal-on" approaches for detection of MicroRNAs.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2021.114356
Abstract: A dual-signaling electrochemical ratio metric strategy was developed for detection microRNA-18a based on the duplex-specific nuclease-assisted target recycling and electrochemical atom transfer radical polymerization signal amplification. In the presence of target microRNA, RNA/DNA duplexes are… read more here.
Keywords: detection; target; duplex specific; signaling electrochemical ... See more keywords
Duplex-specific nuclease assisted miRNA assay based on gold and silver nanoparticles co-decorated on electrode interface.
Sign Up to like & getrecommendations! Published in 2020 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2020.01.041
Abstract: miRNAs are small non-coding RNAs for gene regulation, which serve as promising biomarkers for the diagnosis of certain diseases. In this contribution, we have proposed a convenient electrochemical biosensing strategy based on the interaction between… read more here.
Keywords: silver nanoparticles; decorated electrode; dna; specific nuclease ... See more keywords
Signal amplification method for miR-205 assay through combining graphene oxide with duplex-specific nuclease
Sign Up to like & getrecommendations! Published in 2019 at "RSC Advances"
DOI: 10.1039/c9ra04663a
Abstract: A signal amplified method for detecting a biomarker of radiation-resistant nasopharyngeal carcinoma using graphene oxide and duplex-specific nuclease was constructed. read more here.
Keywords: graphene oxide; specific nuclease; oxide duplex; duplex specific ... See more keywords
Dual signal amplification for microRNA-21 detection based on duplex-specific nuclease and invertase
Sign Up to like & getrecommendations! Published in 2020 at "RSC Advances"
DOI: 10.1039/c9ra10657j
Abstract: A portable and sensitive method based on duplex-specific nuclease and MBs–DNA–Inv conjugate has been developed for miRNA-21 detection by using a personal glucose meter as the signal collector. read more here.
Keywords: detection; dual signal; specific nuclease; duplex specific ... See more keywords
State-of-the-Art Fluorescent Probes: Duplex-Specific Nuclease-Based Strategies for Early Disease Diagnostics
Sign Up to like & getrecommendations! Published in 2022 at "Biosensors"
DOI: 10.3390/bios12121172
Abstract: Precision healthcare aims to improve patient health by integrating prevention measures with early disease detection for prompt treatments. For the delivery of preventive healthcare, cutting-edge diagnostics that enable early disease detection must be clinically adopted.… read more here.
Keywords: specific nuclease; disease; detection; early disease ... See more keywords
SARS-CoV-2 RNA Detection with Duplex-Specific Nuclease Signal Amplification
Sign Up to like & getrecommendations! Published in 2021 at "Micromachines"
DOI: 10.3390/mi12020197
Abstract: The emergence of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a zoonotic pathogen, has led to the outbreak of coronavirus disease 2019 (COVID-19) pandemic and brought serious threats to public health worldwide. The… read more here.
Keywords: detection; specific nuclease; duplex specific; signal amplification ... See more keywords
[Construction of microRNA-21 terahertz metamaterial sensing method based on duplex-specific nuclease triggered rolling circle amplification].
Sign Up to like & getrecommendations! Published in 2021 at "Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]"
DOI: 10.3760/cma.j.cn112150-20201111-01359
Abstract: Objective: To construct of a terahertz metamaterial sensing method for detection of microRNA-21 (miRNA-21) based on duplex-specific nuclease triggered rolling circle amplification. Methods: First, a THz metamaterial sensing method was constructed, and verified by polyacrylamide… read more here.
Keywords: sensing method; rolling circle; specific nuclease; duplex specific ... See more keywords