Rapid differential detection of Genotype I and III Japanese Encephalitis Virus from clinical samples by a novel duplex TaqMan probe-based RT-qPCR assay.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2020.113841
Abstract: Japanese Encephalitis (JE) is an acute infectious disease that threatens both human and pig populations throughout Asia. JE is caused by the Japanese Encephalitis Virus (JEV), of which Genotype III (GIII) had been the most… read more here.
Keywords: jev; taqman; japanese encephalitis; duplex taqman ... See more keywords
Single- and duplex TaqMan-quantitative PCR for determining the copy numbers of integrated selection markers during site-specific mutagenesis in Toxoplasma gondii by CRISPR-Cas9
Sign Up to like & getrecommendations! Published in 2022 at "PLoS ONE"
DOI: 10.1101/2022.06.23.497358
Abstract: Herein, we developed a single and a duplex TaqMan quantitative qPCR for absolute quantification of copy numbers of integrated dihydrofolate reductase-thymidylate synthase (mdhfr-ts) drug selectable marker for pyrimethamine resistance in Toxoplasma gondii knockouts (KOs). The… read more here.
Keywords: crispr cas9; duplex taqman; taqman; toxoplasma ... See more keywords
Rapid Differential Detection of Japanese Encephalitis Virus and Getah Virus in Pigs or Mosquitos by a Duplex TaqMan Real-Time RT-PCR Assay
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Veterinary Science"
DOI: 10.3389/fvets.2022.839443
Abstract: Both JEV (Japanese encephalitis virus) and GETV (Getah virus) pose huge threats to the safety of animals and public health. Pigs and mosquitoes play a primary role in JEV and GETV transmission. However, there is… read more here.
Keywords: duplex taqman; detection; getv; jev getv ... See more keywords