Targeted Gene Knockdown in Early Embryos Using siRNA.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6988-3_14
Abstract: RNA interference is a convenient and highly effective technique to investigate the biological function of genes. Adequately designed RNA molecules introduced into an oocyte are able to bind specific endogenous mRNAs and trigger their degradation.… read more here.
Keywords: embryos using; using sirna; early embryos; gene ... See more keywords
Superovulation alters DNA methyltransferase protein expression in mouse oocytes and early embryos
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Assisted Reproduction and Genetics"
DOI: 10.1007/s10815-017-1087-z
Abstract: PurposeDNA methylation is an epigenetic mechanism that plays critical roles during mammalian oocyte and preimplantation embryo development. It is achieved by adding a methyl group to the fifth carbon atom of cytosine residues within cytosine-phosphate-guanine… read more here.
Keywords: mouse oocytes; oocytes early; superovulation; expression ... See more keywords
Insights into epigenetic patterns in mammalian early embryos
Sign Up to like & getrecommendations! Published in 2020 at "Protein & Cell"
DOI: 10.1007/s13238-020-00757-z
Abstract: Mammalian fertilization begins with the fusion of two specialized gametes, followed by major epigenetic remodeling leading to the formation of a totipotent embryo. During the development of the pre-implantation embryo, precise reprogramming progress is a… read more here.
Keywords: epigenetic patterns; embryo; insights epigenetic; mammalian early ... See more keywords
Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2018.03.036
Abstract: Microinjection is an essential approach in the study of mammalian oocytes and early embryos, and is useful for the introduction of many molecules and reagents. Whereas microinjection into germinal vesicle stage oocytes is relatively simple… read more here.
Keywords: electrical assisted; oocytes early; microinjection; early embryos ... See more keywords
Dcaf11 activates Zscan4-mediated alternative telomere lengthening in early embryos and embryonic stem cells.
Sign Up to like & getrecommendations! Published in 2020 at "Cell stem cell"
DOI: 10.1016/j.stem.2020.11.018
Abstract: Telomeres play vital roles in ensuring chromosome stability and are thus closely linked with the onset of aging and human disease. Telomeres undergo extensive lengthening during early embryogenesis. However, the detailed molecular mechanism of telomere… read more here.
Keywords: embryos; embryonic stem; early embryos; stem cells ... See more keywords
Unfolded protein response triggers differential apoptotic mechanisms in ovaries and early embryos exposed to maternal type 1 diabetes
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-92093-3
Abstract: Diabetes mellitus (DM) has profound effects on the female mammalian reproductive system, and early embryonic development, reducing female reproductive outcomes and inducing developmental programming in utero. However, the underlying cellular and molecular mechanisms remain poorly… read more here.
Keywords: unfolded protein; protein response; response triggers; type ... See more keywords
2 The landscape of accessible chromatin in bovine oocytes and early embryos
Sign Up to like & getrecommendations! Published in 2020 at "Reproduction, Fertility and Development"
DOI: 10.1071/rdv32n2ab2
Abstract: Chromatin reorganization governs gene expression regulation during pre-implantation development. However, the global chromatin landscape and its dynamics in this period remain unexplored in bovine. In this study, we constructed a genome-wide map of accessible chromatin… read more here.
Keywords: accessible chromatin; chromatin; bovine; landscape ... See more keywords
Environmental epigenetic interaction of gametes and early embryos
Sign Up to like & getrecommendations! Published in 2022 at "Biology of Reproduction"
DOI: 10.1093/biolre/ioac051
Abstract: Abstract In recent years, the developmental origins of diseases have been increasingly recognized and accepted. As such, it has been suggested that most adulthood chronic diseases such as diabetes, obesity, cardiovascular disease, and even tumors… read more here.
Keywords: epigenetic interaction; environmental epigenetic; early embryos; gametes early ... See more keywords
Bioengineered microenvironment to culture early embryos
Sign Up to like & getrecommendations! Published in 2020 at "Cell Proliferation"
DOI: 10.1111/cpr.12754
Abstract: The abnormalities of early post‐implantation embryos can lead to early pregnancy loss and many other syndromes. However, it is hard to study embryos after implantation due to the limited accessibility. The success of embryo culture… read more here.
Keywords: embryonic development; microenvironment culture; bioengineered microenvironment; early embryos ... See more keywords
New Wenshen Shengjing Decoction Improves Early Embryonic Development by Maintaining Low Levels of H3K4me3 in Sperm
Sign Up to like & getrecommendations! Published in 2022 at "BioMed Research International"
DOI: 10.1155/2022/9775473
Abstract: Background New Wenshen Shengjing Decoction (NWSSJD), a traditional Chinese compound medicine, has significant effect on spermatogenesis disorder and can significantly improve sperm quality. Many components in NWSSJD can induce epigenetic modifications of different types of… read more here.
Keywords: embryonic development; development; embryos; expression ... See more keywords
De novo assembly of a transcriptome from the eggs and early embryos of Astropecten aranciacus
Sign Up to like & getrecommendations! Published in 2017 at "PLoS ONE"
DOI: 10.1371/journal.pone.0184090
Abstract: Starfish have been instrumental in many fields of biological and ecological research. Oocytes of Astropecten aranciacus, a common species native to the Mediterranean Sea and the East Atlantic, have long been used as an experimental… read more here.
Keywords: novo assembly; transcriptome eggs; early embryos; assembly transcriptome ... See more keywords