A safe an easy method for building consensus HIV sequences from 454 massively parallel sequencing data.
Sign Up to like & getrecommendations! Published in 2018 at "Enfermedades Infecciosas Y Microbiologia Clinica"
DOI: 10.1016/j.eimce.2016.08.004
Abstract: Abstract Objective To show how to generate a consensus sequence from the information of massive parallel sequences data obtained from routine HIV anti-retroviral resistance studies, and that may be suitable for molecular epidemiology studies. Material… read more here.
Keywords: safe easy; hiv; consensus; median bootstrap ... See more keywords
An ‘easy’ method to understand perianal sepsis – a video vignette
Sign Up to like & getrecommendations! Published in 2019 at "Colorectal Disease"
DOI: 10.1111/codi.14782
Abstract: Perianal sepsis is one of the most challenging diseases for the colorectal surgeon. The management of this pathology can be associated with high morbidity. Incorrect diagnosis is quite common with consequent improper drainage. This leads… read more here.
Keywords: sepsis; understand perianal; perianal sepsis; method understand ... See more keywords
Easy method for six-fragment Golden Gate Assembly of modular vectors.
Sign Up to like & getrecommendations! Published in 2023 at "BioTechniques"
DOI: 10.2144/btn-2022-0041
Abstract: Efficient cloning techniques are a requirement for synthetic biology. This study provides a simplified cloning method based on Golden Gate Assembly that can be used for rapid vector construction. The building of multiple expression vectors… read more here.
Keywords: easy method; golden gate; gate assembly; method six ... See more keywords