Persistence of CRISPR/Cas9 gene edited hematopoietic stem cells following transplantation: A systematic review and meta‐analysis of preclinical studies
Sign Up to like & getrecommendations! Published in 2021 at "Stem Cells Translational Medicine"
DOI: 10.1002/sctm.20-0520
Abstract: Gene editing blood‐derived cells is an attractive approach to cure selected monogenic diseases but remains experimental. A systematic search of preclinical controlled studies is needed to determine the persistence of edited cells following reinfusion. All… read more here.
Keywords: crispr cas9; edited cells; gene edited; gene ... See more keywords
Off-target genome editing: A new discipline of gene science and a new class of medicine
Sign Up to like & getrecommendations! Published in 2019 at "Cell Biology and Toxicology"
DOI: 10.1007/s10565-019-09475-7
Abstract: With an increasing growth of genome editing, off-target effects such as non-specific genetic modifications resulting from the designed process of genome editing become a new discipline of gene science and new class medicine. The degree… read more here.
Keywords: genome editing; medicine; target effects; edited cells ... See more keywords
Safety and feasibility of CRISPR-edited T cells in patients with refractory non-small-cell lung cancer
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DOI: 10.1038/s41591-020-0840-5
Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR)–Cas9 editing of immune checkpoint genes could improve the efficacy of T cell therapy, but the first necessary undertaking is to understand the safety and feasibility. Here, we report… read more here.
Keywords: crispr; edited cells; safety feasibility; lung cancer ... See more keywords
CRISPRroots: on- and off-target assessment of RNA-seq data in CRISPR–Cas9 edited cells
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab1131
Abstract: Abstract The CRISPR-Cas9 genome editing tool is used to study genomic variants and gene knockouts, and can be combined with transcriptomic analyses to measure the effects of such alterations on gene expression. But how can… read more here.
Keywords: cas9; seq data; rna seq; edited cells ... See more keywords
Identification of genome edited cells using CRISPRnano
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac440
Abstract: Abstract Genome engineering-induced cleavage sites can be resolved by non-homologous end joining (NHEJ) or homology-directed repair (HDR). Identifying genetically modified clones at the target locus remains an intensive and laborious task. Different workflows and software… read more here.
Keywords: cells using; identification genome; genome edited; identification ... See more keywords