Whole-genome sequencing reveals rare off-target mutations in CRISPR/Cas9-edited grapevine
Sign Up to like & getrecommendations! Published in 2021 at "Horticulture Research"
DOI: 10.1038/s41438-021-00549-4
Abstract: The CRISPR (clustered regularly interspaced short palindromic repeats)-associated protein 9 (Cas9) system is a powerful tool for targeted genome editing, with applications that include plant biotechnology and functional genomics research. However, the specificity of Cas9… read more here.
Keywords: crispr cas9; target; edited grapevine; cas9 edited ... See more keywords
Regeneration of non-chimeric plants from DNA-free edited grapevine protoplasts
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.1078931
Abstract: The application of New Breeding Techniques (NBTs) in Vitis vinifera is highly desirable to introduce valuable traits while preserving the genotype of the elite cultivars. However, a broad application of NBTs through standard DNA-based transformation… read more here.
Keywords: chimeric plants; dna free; grapevine; edited grapevine ... See more keywords