Pioneer Factor Improves CRISPR‐Based C‐To‐G and C‐To‐T Base Editing
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Science"
DOI: 10.1002/advs.202202957
Abstract: Base editing events in eukaryote require a compatible chromatin environment, but there is little research on how chromatin factors contribute to the editing efficiency or window. By engineering BEs (base editors) fused with various pioneer… read more here.
Keywords: editing efficiency; pioneer; base editing; efficiency ... See more keywords
Precise A∙T to G∙C base editing in the allotetraploid rapeseed (Brassica napus L.) genome
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Cellular Physiology"
DOI: 10.1002/jcp.30904
Abstract: Rapeseed is an important source of oilseed crop in the world. Achieving genetic improvement has always been the major goal in rapeseed production. Single nucleotide substitution is the basis of most genetic variation underpinning important… read more here.
Keywords: editing efficiency; precise base; brassica napus; rapeseed ... See more keywords
Expanded targeting scope and enhanced base editing efficiency in rabbit using optimized xCas9(3.7)
Sign Up to like & getrecommendations! Published in 2019 at "Cellular and Molecular Life Sciences"
DOI: 10.1007/s00018-019-03110-8
Abstract: Evolved xCas9(3.7) variant with broad PAM compatibility has been reported in cell lines, while its editing efficiency was site-specific. Here, we show that xCas9(3.7) can recognize a broad PAMs including NGG, NGA, and NGT, in… read more here.
Keywords: base editing; optimized xcas9; base; editing efficiency ... See more keywords
GFP tagging based method to analyze the genome editing efficiency of CRISPR/Cas9-gRNAs through transient expression in N. benthamiana
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Plant Biochemistry and Biotechnology"
DOI: 10.1007/s13562-019-00540-0
Abstract: The CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats/CRISPR—associated proteins 9) is simple and highly efficient technology applied to functional studies of genes and genetic crop improvement. In this study, we have demonstrated the utility of… read more here.
Keywords: genome editing; editing efficiency; crispr cas9; gfp ... See more keywords
Efficient Polymer-Mediated Delivery of Gene-Editing Ribonucleoprotein Payloads through Combinatorial Design, Parallelized Experimentation, and Machine Learning.
Sign Up to like & getrecommendations! Published in 2020 at "ACS nano"
DOI: 10.1021/acsnano.0c08549
Abstract: Chemically defined vectors such as cationic polymers are versatile alternatives to engineered viruses for the delivery of genome-editing payloads. However, their clinical translation hinges on rapidly exploring vast chemical design spaces and deriving structure-function relationships… read more here.
Keywords: editing efficiency; machine learning; delivery; design parallelized ... See more keywords
Engineered pegRNAs improve prime editing efficiency.
Sign Up to like & getrecommendations! Published in 2021 at "Nature biotechnology"
DOI: 10.1038/s41587-021-01039-7
Abstract: Prime editing enables the installation of virtually any combination of point mutations, small insertions or small deletions in the DNA of living cells. A prime editing guide RNA (pegRNA) directs the prime editor protein to… read more here.
Keywords: editing efficiency; engineered pegrnas; improve prime; pegrnas improve ... See more keywords
DeepCRISTL: deep transfer learning to predict CRISPR/Cas9 functional and endogenous on-target editing efficiency
Sign Up to like & getrecommendations! Published in 2022 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btac218
Abstract: Abstract Motivation CRISPR/Cas9 technology has been revolutionizing the field of gene editing in recent years. Guide RNAs (gRNAs) enable Cas9 proteins to target specific genomic loci for editing. However, editing efficiency varies between gRNAs. Thus,… read more here.
Keywords: deepcristl; editing efficiency; high throughput; functional endogenous ... See more keywords
Optimization of CRISPR/LbCas12a-mediated gene editing in Arabidopsis
Sign Up to like & getrecommendations! Published in 2022 at "PLoS ONE"
DOI: 10.1371/journal.pone.0265114
Abstract: CRISPR/LbCas12a system (LbCpf1) has been widely used for genome modification including plant species. However, the efficiency of CRISPR/LbCas12a varied considerably in different plant species and tissues, and the editing efficiency needs to be further improved.… read more here.
Keywords: editing efficiency; crispr; efficiency crispr; crispr lbcas12a ... See more keywords
Salt stress affects mRNA editing in soybean chloroplasts
Sign Up to like & getrecommendations! Published in 2017 at "Genetics and Molecular Biology"
DOI: 10.1590/1678-4685-gmb-2016-0055
Abstract: Abstract Soybean, a crop known by its economic and nutritional importance, has been the subject of several studies that assess the impact and the effective plant responses to abiotic stresses. Salt stress is one of… read more here.
Keywords: editing efficiency; salt; salt stress; stress affects ... See more keywords
Establishment of an Efficient Genome Editing System in Lettuce Without Sacrificing Specificity
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.930592
Abstract: The efficiency of the CRISPR/Cas9 genome editing system remains limited in many crops. Utilizing strong promoters to boost the expression level of Cas9 are commonly used to improve the editing efficiency. However, these strategies also… read more here.
Keywords: genome editing; editing efficiency; cas9; editing system ... See more keywords
ABE8e with Polycistronic tRNA-gRNA Expression Cassette Sig-Nificantly Improves Adenine Base Editing Efficiency in Nicotiana benthamiana
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms22115663
Abstract: Adenine base editor containing TadA8e (ABE8e) has been reported in rice. However, the application of ABE8e in other plant species has not been described, and the comparison between ABE8e and ABE7.10, which is widely used… read more here.
Keywords: abe8e; ptg; editing efficiency; benthamiana ... See more keywords