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   Articles with "editing efficiency" as a keyword



Pioneer Factor Improves CRISPR‐Based C‐To‐G and C‐To‐T Base Editing

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recommendations! Published in 2022 at "Advanced Science"

DOI: 10.1002/advs.202202957

Abstract: Base editing events in eukaryote require a compatible chromatin environment, but there is little research on how chromatin factors contribute to the editing efficiency or window. By engineering BEs (base editors) fused with various pioneer… read more here.

Keywords: editing efficiency; pioneer; base editing; efficiency ... See more keywords

Engineering of Zinc Finger Nucleases Through Structural Modeling Improves Genome Editing Efficiency in Cells

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recommendations! Published in 2024 at "Advanced Science"

DOI: 10.1002/advs.202310255

Abstract: Genome Editing is widely used in biomedical research and medicine. Zinc finger nucleases (ZFNs) are smaller in size than transcription activator‐like effector (TALE) nucleases (TALENs) and CRISPR‐Cas9. Therefore, ZFN‐encoding DNAs can be easily packaged into… read more here.

Keywords: genome editing; zinc finger; zfns; editing efficiency ... See more keywords

Enhancing Prime Editing Efficiency Through Modulation of Methylation on the Newly Synthesized DNA Strand and Prolonged Expression

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recommendations! Published in 2025 at "Advanced Science"

DOI: 10.1002/advs.202417790

Abstract: Prime editors (PEs) have emerged as transformative tools for precision genome engineering, yet their broader application remains constrained by incomplete understanding of repair mechanisms. In this study, it is found that an increase in the… read more here.

Keywords: editing efficiency; methylation; efficiency; prime editing ... See more keywords

Precise A∙T to G∙C base editing in the allotetraploid rapeseed (Brassica napus L.) genome

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recommendations! Published in 2022 at "Journal of Cellular Physiology"

DOI: 10.1002/jcp.30904

Abstract: Rapeseed is an important source of oilseed crop in the world. Achieving genetic improvement has always been the major goal in rapeseed production. Single nucleotide substitution is the basis of most genetic variation underpinning important… read more here.

Keywords: editing efficiency; precise base; brassica napus; rapeseed ... See more keywords

Expanded targeting scope and enhanced base editing efficiency in rabbit using optimized xCas9(3.7)

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recommendations! Published in 2019 at "Cellular and Molecular Life Sciences"

DOI: 10.1007/s00018-019-03110-8

Abstract: Evolved xCas9(3.7) variant with broad PAM compatibility has been reported in cell lines, while its editing efficiency was site-specific. Here, we show that xCas9(3.7) can recognize a broad PAMs including NGG, NGA, and NGT, in… read more here.

Keywords: base editing; optimized xcas9; base; editing efficiency ... See more keywords

GFP tagging based method to analyze the genome editing efficiency of CRISPR/Cas9-gRNAs through transient expression in N. benthamiana

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recommendations! Published in 2019 at "Journal of Plant Biochemistry and Biotechnology"

DOI: 10.1007/s13562-019-00540-0

Abstract: The CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats/CRISPR—associated proteins 9) is simple and highly efficient technology applied to functional studies of genes and genetic crop improvement. In this study, we have demonstrated the utility of… read more here.

Keywords: genome editing; editing efficiency; crispr cas9; gfp ... See more keywords
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Efficient Polymer-Mediated Delivery of Gene-Editing Ribonucleoprotein Payloads through Combinatorial Design, Parallelized Experimentation, and Machine Learning.

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recommendations! Published in 2020 at "ACS nano"

DOI: 10.1021/acsnano.0c08549

Abstract: Chemically defined vectors such as cationic polymers are versatile alternatives to engineered viruses for the delivery of genome-editing payloads. However, their clinical translation hinges on rapidly exploring vast chemical design spaces and deriving structure-function relationships… read more here.

Keywords: editing efficiency; machine learning; delivery; design parallelized ... See more keywords

Engineered pegRNAs improve prime editing efficiency.

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recommendations! Published in 2021 at "Nature biotechnology"

DOI: 10.1038/s41587-021-01039-7

Abstract: Prime editing enables the installation of virtually any combination of point mutations, small insertions or small deletions in the DNA of living cells. A prime editing guide RNA (pegRNA) directs the prime editor protein to… read more here.

Keywords: editing efficiency; engineered pegrnas; improve prime; pegrnas improve ... See more keywords

AI-guided Cas9 engineering provides an effective strategy to enhance base editing

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recommendations! Published in 2025 at "Molecular Systems Biology"

DOI: 10.1038/s44320-025-00142-0

Abstract: Precise genome editing is crucial for functional studies and therapies. Base editors, while powerful, require optimization for efficiency. Meanwhile, emerging protein design methods and protein language models have driven efficient and intelligent protein engineering. In… read more here.

Keywords: editing efficiency; efficiency; promep; engineering ... See more keywords

Graph-CRISPR: a gene editing efficiency prediction model based on graph neural network with integrated sequence and secondary structure feature extraction

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recommendations! Published in 2025 at "Briefings in Bioinformatics"

DOI: 10.1093/bib/bbaf410

Abstract: Abstract Clustered regularly interspaced short palindromic repeats (CRISPR) gene-editing technology has transformed molecular biology. Predicting editing efficiency is crucial for optimization, and numerous computational models have been created. However, many current models struggle to generalize… read more here.

Keywords: editing efficiency; secondary structure; graph crispr; crispr ... See more keywords

DeepCRISTL: deep transfer learning to predict CRISPR/Cas9 functional and endogenous on-target editing efficiency

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recommendations! Published in 2022 at "Bioinformatics"

DOI: 10.1093/bioinformatics/btac218

Abstract: Abstract Motivation CRISPR/Cas9 technology has been revolutionizing the field of gene editing in recent years. Guide RNAs (gRNAs) enable Cas9 proteins to target specific genomic loci for editing. However, editing efficiency varies between gRNAs. Thus,… read more here.

Keywords: deepcristl; editing efficiency; high throughput; functional endogenous ... See more keywords