PrimeDesign software for rapid and simplified design of prime editing guide RNAs.
Sign Up to like & getrecommendations! Published in 2021 at "Nature communications"
DOI: 10.1038/s41467-021-21337-7
Abstract: Prime editing (PE) is a versatile genome editing technology, but design of the required guide RNAs is more complex than for standard CRISPR-based nucleases or base editors. Here we describe PrimeDesign, a user-friendly, end-to-end web… read more here.
Keywords: prime editing; guide rnas; editing guide; design ... See more keywords
Predicting the efficiency of prime editing guide RNAs in human cells
Sign Up to like & getrecommendations! Published in 2020 at "Nature Biotechnology"
DOI: 10.1038/s41587-020-0677-y
Abstract: Prime editing enables the introduction of virtually any small-sized genetic change without requiring donor DNA or double-strand breaks. However, evaluation of prime editing efficiency requires time-consuming experiments, and the factors that affect efficiency have not… read more here.
Keywords: guide rnas; editing guide; prime editing; efficiency ... See more keywords
SynDesign: web-based prime editing guide RNA design and evaluation tool for saturation genome editing
Sign Up to like & getrecommendations! Published in 2024 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkae304
Abstract: Abstract Saturation genome editing (SGE) enables in-depth functional evaluation of disease-associated genes and variants by generating all possible single nucleotide variants (SNVs) within a given coding region. Although prime editing can be employed for inducing… read more here.
Keywords: saturation genome; editing guide; prime editing; genome editing ... See more keywords