CRISPR-Cas systems for genome editing of mammalian cells.
Sign Up to like & getrecommendations! Published in 2021 at "Progress in molecular biology and translational science"
DOI: 10.1016/bs.pmbts.2021.01.011
Abstract: In the past decade, ZFNs and TALENs have been used for targeted genome engineering and have gained scientific attention. It has demonstrated huge potential for gene knockout, knock-in, and indels in desired locations of genomes… read more here.
Keywords: mammalian cells; genome editing; crispr cas; crispr cas9 ... See more keywords
Enhanced Cas12a editing in mammalian cells and zebrafish
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkz184
Abstract: Abstract Type V CRISPR–Cas12a systems provide an alternate nuclease platform to Cas9, with potential advantages for specific genome editing applications. Here we describe improvements to the Cas12a system that facilitate efficient targeted mutagenesis in mammalian… read more here.
Keywords: editing mammalian; cas12a editing; enhanced cas12a; cells zebrafish ... See more keywords