Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning
Sign Up to like & getrecommendations! Published in 2020 at "Cell"
DOI: 10.1016/j.cell.2020.05.037
Abstract: Although base editors are widely used to install targeted point mutations, the factors that determine base editing outcomes are not well understood. We characterized sequence-activity relationships of 11 cytosine and adenine base editors (CBEs and… read more here.
Keywords: base editing; editing outcomes; machine learning; base editors ... See more keywords
Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos
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DOI: 10.1038/s41598-020-71412-0
Abstract: CRISPR/Cas9 genome editing has revolutionized functional genomics in vertebrates. However, CRISPR/Cas9 edited F0 animals too often demonstrate variable phenotypic penetrance due to the mosaic nature of editing outcomes after double strand break (DSB) repair. Even… read more here.
Keywords: editing outcomes; crispr cas9; outcomes xenopus; penetrance ... See more keywords
Annotation and evaluation of base editing outcomes in multiple cell types using CRISPRbase
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DOI: 10.1093/nar/gkac967
Abstract: Abstract CRISPR-Cas base editing (BE) system is a powerful tool to expand the scope and efficiency of genome editing with single-nucleotide resolution. The editing efficiency, product purity, and off-target effect differ among various BE systems.… read more here.
Keywords: crisprbase; cell types; editing outcomes; base editing ... See more keywords