SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds
Sign Up to like & getrecommendations! Published in 2020 at "Genome Biology"
DOI: 10.1186/s13059-020-02051-x
Abstract: We describe here a CRISPR simultaneous and wide-editing induced by a single system (SWISS), in which RNA aptamers engineered in crRNA scaffold recruit their cognate binding proteins fused with cytidine deaminase and adenosine deaminase to… read more here.
Keywords: genome editing; crispr; editing plants; rna ... See more keywords
Base Editing in Plants: Applications, Challenges, and Future Prospects
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2021.664997
Abstract: The ability to create targeted modifications in the genomes of plants using genome editing technologies has revolutionized research in crop improvement in the current dispensation of molecular biology. This technology has attracted global attention and… read more here.
Keywords: base editing; plants applications; editing plants; editing technologies ... See more keywords
Challenges Facing CRISPR/Cas9-Based Genome Editing in Plants
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.902413
Abstract: The development of plant varieties with desired traits is imperative to ensure future food security. The revolution of genome editing technologies based on the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated nuclease 9 (Cas9) system… read more here.
Keywords: genome editing; crispr cas9; editing plants; plant ... See more keywords
Editing plants for virus resistance using CRISPR-Cas.
Sign Up to like & getrecommendations! Published in 2017 at "Acta virologica"
DOI: 10.4149/av_2017_02_02
Abstract: This minireview summarizes recent advancements using the clustered regularly interspaced palindromic repeats-associated nuclease systems (CRISPR-Cas) derived from prokaryotes to breed plants resistant to DNA and RNA viruses. The CRISPR-Cas system represents a powerful tool able… read more here.
Keywords: virus resistance; crispr cas; cas; editing plants ... See more keywords