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Published in 2020 at "Theriogenology"
DOI: 10.1016/j.theriogenology.2020.01.036
Abstract: Historically, genetic engineering in livestock proved to be challenging. Without stable embryonic stem cell lines to utilize, somatic cell nuclear transfer (SCNT) had to be employed to produce many of the genetically engineered (GE) livestock…
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Keywords:
livestock models;
genome editing;
current progress;
livestock ... See more keywords
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2
Published in 2023 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00836
Abstract: Various genome editing tools have been developed for programmable genome manipulation at specified genomic loci. However, it is crucial to comprehensively interrogate the off-target effect induced by these genome editing tools, especially when apply them…
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Keywords:
genome editing;
chemical biological;
editing tools;
interrogate target ... See more keywords
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Published in 2021 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2021.654058
Abstract: Corynebacterium glutamicum has been considered a promising synthetic biological platform for biomanufacturing and bioremediation. However, there are still some challenges in genetic manipulation of C. glutamicum. Recently, more and more genetic parts or elements (replicons,…
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Keywords:
corynebacterium glutamicum;
glutamicum;
genetic manipulation;
editing tools ... See more keywords
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Published in 2022 at "Frontiers in Neuroscience"
DOI: 10.3389/fnins.2022.924917
Abstract: Gene editing and gene regulatory fields are continuously developing new and safer tools that move beyond the initial CRISPR/Cas9 technology. As more advanced applications are emerging, it becomes crucial to understand and establish more complex…
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Keywords:
gene regulatory;
gene;
proof concept;
gene editing ... See more keywords
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Published in 2022 at "Biology"
DOI: 10.3390/biology11060862
Abstract: Simple Summary β-thalassemia syndromes are clinically and genetically heterogeneous blood disorders presented by β-chain deficiency in hemoglobin production. Despite improvements in transfusion practices and chelation treatment, many lingering challenges have encouraged researchers to develop newer…
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Keywords:
editing based;
gene editing;
thalassemia;
editing tools ... See more keywords
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Published in 2021 at "Genes"
DOI: 10.3390/genes12060797
Abstract: Genome-editing (GE) is having a tremendous influence around the globe in the life science community. Among its versatile uses, the desired modifications of genes, and more importantly the transgene (DNA)-free approach to develop genetically modified…
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Keywords:
genome editing;
crispr based;
insights technological;
editing tools ... See more keywords
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Published in 2023 at "Genes"
DOI: 10.3390/genes14020483
Abstract: Programmable nucleases, such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas, are widely accepted because of their diversity and enormous potential for targeted genomic modifications…
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Keywords:
genome editing;
progress prospects;
human diseases;
editing tools ... See more keywords
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3
Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23031145
Abstract: Rapid assessment of clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas)-based genome editing (GE) tools and their components is a critical aspect for successful GE applications in different organisms. In many bacteria, double-strand breaks (DSBs)…
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Keywords:
editing tools;
crispr;
base;
base editing ... See more keywords